Immunodiffusion
From Wikipedia, the free encyclopedia
From Wikipedia, the free encyclopedia
Immunodiffusion is a laboratory technique used to detect and quantify antigens and antibodies by observing their interactions within a gel medium.[1] This technique involves the diffusion of antigens and antibodies through a gel, usually agar, resulting in the formation of a visible precipitate when they interact.[1][2]
Immunodiffusion techniques are widely used in immunology for various purposes, including:[1][2]
In this method, antibodies are uniformly distributed in an agar gel, and the antigen sample is placed in wells cut into the gel. As the antigen diffuses radially, it forms a precipitation ring with the antibody. The diameter of this ring corresponds to the concentration of the antigen in the solution.[3][2]
This method involves both antigen and antibody diffusing through the gel from separate wells, forming precipitation lines where they meet and react.[4]
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