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DNA gel stain for molecular genetics From Wikipedia, the free encyclopedia
GelGreen is an intercalating nucleic acid stain used in molecular genetics for agarose gel DNA electrophoresis. GelGreen consists of two acridine orange subunits that are bridged by a linear oxygenated spacer.[1][2]
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Preferred IUPAC name
10,10′-(6,22-Dioxo-11,14,17-trioxa-7,21-diazaheptacosane-1,27-diyl)bis[3,6-bis(dimethylamino)acridin-10-ium] diiodide | |
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CompTox Dashboard (EPA) |
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Properties | |
C56H80I2N8O5 | |
Molar mass | 1198.43 g/mol |
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NFPA 704 (fire diamond) | |
Flash point | > 100 °C (212 °F; 373 K) |
Safety data sheet (SDS) | 10,000X in water, Biotium Inc. |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Its fluorophore, and therefore its optical properties, are essentially identical to those of other N-alkylacridinium orange dyes. When exposed to ultraviolet light, it will fluoresce with a greenish color that strongly intensifies after binding to DNA.[3] The substance is marketed as a less toxic and more sensitive alternative to ethidium bromide.[3] GelGreen is sold as a solution in either DMSO or water.[3]
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