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EcoRI
Restriction enzyme / From Wikipedia, the free encyclopedia
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EcoRI (pronounced "eco R one") is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites, and is also a part of the restriction modification system.[1] The Eco part of the enzyme's name originates from the species from which it was isolated - "E" denotes generic name which is "Escherichia" and "co" denotes species name, "coli" - while the R represents the particular strain, in this case RY13, and the I denotes that it was the first enzyme isolated from this strain.[citation needed]
EcoRI | |||||||||
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Identifiers | |||||||||
Symbol | EcoRI | ||||||||
Pfam | PF02963 | ||||||||
InterPro | IPR004221 | ||||||||
SCOP2 | 1na6 / SCOPe / SUPFAM | ||||||||
CDD | 79lll | ||||||||
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In molecular biology it is used as a restriction enzyme. EcoRI creates 4 nucleotide sticky ends with 5' end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G↓AATTC, which has a palindromic complementary sequence of CTTAA↓G.[2] Other restriction enzymes, depending on their cut sites, can also leave 3' overhangs or blunt ends with no overhangs.