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Chemical compound From Wikipedia, the free encyclopedia
Dichlorofluorescein (DCF) is an organic dye of the fluorescein family, being substituted at the 2 and 7 positions by chloride.
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Preferred IUPAC name
2′,7′-Dichloro-3′,6′-dihydroxy-3H-spiro[[2]benzofuran-1,9′-xanthen]-3-one | |
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ECHA InfoCard | 100.000.881 |
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Properties | |
C20H10Cl2O5 | |
Molar mass | 401.20 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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It is used as an indicator for argentometry by Fajans method.[1][2]
When used as an indicator, upon reaching the equivalence point of a titration reaction the color shifts from colorless towards a faint pink.
It is also used in the cellular antioxidant activity (CAA) assay. Dichlorofluorescin (DCFH) is a probe that is trapped within cells and is easily oxidized to fluorescent dichlorofluorescein (DCF). The method measures the ability of compounds to prevent the formation of DCF by 2,2'-Azobis(2-amidinopropane) dihydrochloride (ABAP)-generated peroxyl radicals in human hepatocarcinoma HepG2 cells.[3] By itself, dichlorofluorescin (DCFH) also quantifies intracellular hydrogen peroxide as well as cellular oxidative stress.[4]
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