Cytochrome P450 aromatic O-demethylase
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Cytochrome P450 aromatic O-demethylase is a bacterial enzyme that catalyzes the demethylation of lignin and various lignols. The net reaction follows the following stoichiometry, illustrated with a generic methoxy arene:[1]
- ArOCH3 + O2 + 2 e− + 2 H+ → ArOH + CH2O + H2O
Quick Facts Aromatic O-demethylase, cytochrome P450 subunit, Identifiers ...
Aromatic O-demethylase, cytochrome P450 subunit | |||||||
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Identifiers | |||||||
Organism | Amycolatopsis sp. | ||||||
Symbol | gcoA | ||||||
PDB | 5NCB | ||||||
UniProt | P0DPQ7 | ||||||
Other data | |||||||
EC number | 1.14.14.- | ||||||
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Quick Facts Aromatic O-demethylase, reductase subunit, Identifiers ...
Aromatic O-demethylase, reductase subunit | |||||||
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Identifiers | |||||||
Organism | Amycolatopsis sp. | ||||||
Symbol | gcoB | ||||||
PDB | 5OGX | ||||||
UniProt | P0DPQ8 | ||||||
Other data | |||||||
EC number | 1.6.2.- | ||||||
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The enzyme is notable for its promiscuity, affecting the O-demethylation of a range of substrates, including lignin.
It is a heterodimeric protein derived from the products of two genes. The component proteins are a cytochrome P450 enzyme (encoded by the gcoA gene from the family CYP255A) and a three-domain reductase (encoded by the gcoB gene) complexed with three cofactors (2Fe-2S, FAD, and NADH).[1]