CD43

Leukosialin also known as sialophorin or CD43 (cluster of differentiation 43) is a transmembrane cell surface protein that in humans is encoded by the SPN (sialophorin) gene.^[5][6][7]

SPN
Identifiers
Aliases	SPN, CD43, GALGP, GPL115, LSN, sialophorin, LEU-22
External IDs	OMIM: 182160; MGI: 98384; HomoloGene: 36108; GeneCards: SPN; OMA:SPN - orthologs
Gene location (Human) Chr. Chromosome 16 (human)[1] Band 16p11.2 Start 29,662,979 bp[1] End 29,670,876 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7 F3|7 69.35 cM Start 126,731,404 bp[2] End 126,736,995 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in buccal mucosa cell granulocyte monocyte tendon of biceps brachii sperm pancreatic ductal cell bone marrow cells vena cava triceps brachii muscle internal globus pallidus Top expressed in blood granulocyte thymus lymph node mesenteric lymph nodes spleen decidua tibiofemoral joint body of femur submandibular gland More reference expression data BioGPS n/a
Gene ontology Molecular function transmembrane signaling receptor activity Hsp70 protein binding heat shock protein binding Cellular component integral component of membrane membrane plasma membrane integral component of plasma membrane basement membrane cell surface uropod extracellular exosome external side of plasma membrane extracellular space Biological process negative regulation of cell adhesion positive regulation of protein phosphorylation negative regulation of type IV hypersensitivity response to protozoan T cell costimulation negative thymic T cell selection chemotaxis establishment or maintenance of cell polarity cellular defense response cell surface receptor signaling pathway negative regulation of T cell proliferation defense response to bacterium immune response positive regulation of T cell proliferation regulation of immune response apoptotic signaling pathway regulation of defense response to virus negative regulation of T cell activation leukocyte migration signal transduction Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 6693 20737 Ensembl ENSG00000197471 ENSMUSG00000051457 UniProt P16150 P15702 RefSeq (mRNA) NM_001030288 NM_003123 NM_001037810 NM_009259 RefSeq (protein) NP_001025459 NP_003114 NP_001032899 NP_033285 Location (UCSC) Chr 16: 29.66 – 29.67 Mb Chr 7: 126.73 – 126.74 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Function

Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.^[5]

Clinical significance

Defects in the CD43 molecule are associated with the development of Wiskott–Aldrich syndrome.^[8] It also appears in about 25% of intestinal MALTomas.^{[citation needed]} Using immunohistochemistry, CD43 can be demonstrated in the paracortical T-cells of healthy lymph nodes and tonsils; it is also positive in a range of lymphoid and myeloid tumours. Although it is present in over 90% of T-cell lymphomas, it is generally less effective at demonstrating this condition than is CD3 antigen. However, it may be useful as part of a panel to demonstrate B-cell lymphoblastic lymphoma, since the malignant cells in this condition are often CD43 positive, and may be difficult to stain with other antibodies. Because it stains granulocytes and their precursors, it is also an effective marker for myeloid tumours.^[9]

Interactions

CD43 has been shown to interact with EZR^[10] and Moesin.^[10][11]

References

1. GRCh38: Ensembl release 89: ENSG00000197471 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000051457 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: SPN sialophorin (leukosialin, CD43)".
6. Pallant A, Eskenazi A, Mattei MG, Fournier RE, Carlsson SR, Fukuda M, Frelinger JG (February 1989). "Characterization of cDNAs encoding human leukosialin and localization of the leukosialin gene to chromosome 16". Proc. Natl. Acad. Sci. U.S.A. 86 (4): 1328–32. Bibcode:1989PNAS...86.1328P. doi:10.1073/pnas.86.4.1328. PMC 286683. PMID 2521952.
7. Shelley CS, Remold-O'Donnell E, Davis AE, Bruns GA, Rosen FS, Carroll MC, Whitehead AS (April 1989). "Molecular characterization of sialophorin (CD43), the lymphocyte surface sialoglycoprotein defective in Wiskott-Aldrich syndrome". Proc. Natl. Acad. Sci. U.S.A. 86 (8): 2819–23. Bibcode:1989PNAS...86.2819S. doi:10.1073/pnas.86.8.2819. PMC 287010. PMID 2784859.
8. Remold-O'Donnell E, Rosen FS (1990). "Sialophorin (CD43) and the Wiskott–Aldrich syndrome". Immunodefic Rev. 2 (2): 151–74. PMID 2223062.
9. Leong, Anthony S-Y, Cooper, Kumarason, Leong, F Joel W-M (2003). Manual of Diagnostic Cytology (2nd ed.). Greenwich Medical Media, Ltd. p. 113. ISBN 978-1-84110-100-2.
10. Serrador JM, Nieto M, Alonso-Lebrero JL, del Pozo MA, Calvo J, Furthmayr H, Schwartz-Albiez R, Lozano F, González-Amaro R, Sánchez-Mateos P, Sánchez-Madrid F (June 1998). "CD43 interacts with moesin and ezrin and regulates its redistribution to the uropods of T lymphocytes at the cell-cell contacts". Blood. 91 (12): 4632–44. doi:10.1182/blood.V91.12.4632. PMID 9616160.
11. Yonemura S, Hirao M, Doi Y, Takahashi N, Kondo T, Tsukita S, Tsukita S (February 1998). "Ezrin/radixin/moesin (ERM) proteins bind to a positively charged amino acid cluster in the juxta-membrane cytoplasmic domain of CD44, CD43, and ICAM-2". J. Cell Biol. 140 (4): 885–95. doi:10.1083/jcb.140.4.885. PMC 2141743. PMID 9472040.

Further reading

• Fukuda M, Carlsson SR (1987). "Leukosialin, a major sialoglycoprotein on human leukocytes as differentiation antigens". Med. Biol. 64 (6): 335–43. PMID 2950285.
• Rogaev EI, Keryanov SA (1993). "Unusual variability of the complex dinucleotide repeat block at the SPN locus". Hum. Mol. Genet. 1 (8): 657. doi:10.1093/hmg/1.8.657. PMID 1301183.
• Rosenstein Y, Park JK, Hahn WC, et al. (1992). "CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1". Nature. 354 (6350): 233–5. Bibcode:1991Natur.354..233R. doi:10.1038/354233a0. PMID 1683685. S2CID 4256329.
• Schmid K, Hediger MA, Brossmer R, et al. (1992). "Amino acid sequence of human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin)". Proc. Natl. Acad. Sci. U.S.A. 89 (2): 663–7. Bibcode:1992PNAS...89..663S. doi:10.1073/pnas.89.2.663. PMC 48299. PMID 1731338.
• Kudo S, Fukuda M (1991). "A short, novel promoter sequence confers the expression of human leukosialin, a major sialoglycoprotein on leukocytes". J. Biol. Chem. 266 (13): 8483–9. doi:10.1016/S0021-9258(18)93000-0. PMID 1827122.
• Park JK, Rosenstein YJ, Remold-O'Donnell E, et al. (1991). "Enhancement of T-cell activation by the CD43 molecule whose expression is defective in Wiskott-Aldrich syndrome". Nature. 350 (6320): 706–9. Bibcode:1991Natur.350..706P. doi:10.1038/350706a0. PMID 2023632. S2CID 4335838.
• Shelley CS, Remold-O'Donnell E, Rosen FS, Whitehead AS (1990). "Structure of the human sialophorin (CD43) gene. Identification of features atypical of genes encoding integral membrane proteins". Biochem. J. 270 (3): 569–76. doi:10.1042/bj2700569. PMC 1131770. PMID 2241892.
• Srinivas RV, Su T, Trimble LA, et al. (1996). "Enhanced susceptibility to human immunodeficiency virus infection in CD4+ T lymphocytes genetically deficient in CD43". AIDS Res. Hum. Retroviruses. 11 (9): 1015–21. doi:10.1089/aid.1995.11.1015. PMID 8554898.
• Hirao M, Sato N, Kondo T, et al. (1996). "Regulation mechanism of ERM (ezrin/radixin/moesin) protein/plasma membrane association: possible involvement of phosphatidylinositol turnover and Rho-dependent signaling pathway". J. Cell Biol. 135 (1): 37–51. doi:10.1083/jcb.135.1.37. PMC 2121020. PMID 8858161.
• Yonemura S, Hirao M, Doi Y, et al. (1998). "Ezrin/radixin/moesin (ERM) proteins bind to a positively charged amino acid cluster in the juxta-membrane cytoplasmic domain of CD44, CD43, and ICAM-2". J. Cell Biol. 140 (4): 885–95. doi:10.1083/jcb.140.4.885. PMC 2141743. PMID 9472040.
• Pedraza-Alva G, Mérida LB, Burakoff SJ, Rosenstein Y (1998). "T cell activation through the CD43 molecule leads to Vav tyrosine phosphorylation and mitogen-activated protein kinase pathway activation". J. Biol. Chem. 273 (23): 14218–24. doi:10.1074/jbc.273.23.14218. PMID 9603925.
• Serrador JM, Nieto M, Alonso-Lebrero JL, et al. (1998). "CD43 interacts with moesin and ezrin and regulates its redistribution to the uropods of T lymphocytes at the cell-cell contacts". Blood. 91 (12): 4632–44. doi:10.1182/blood.V91.12.4632. PMID 9616160.
• Pace KE, Lee C, Stewart PL, Baum LG (1999). "Restricted receptor segregation into membrane microdomains occurs on human T cells during apoptosis induced by galectin-1". J. Immunol. 163 (7): 3801–11. doi:10.4049/jimmunol.163.7.3801. PMID 10490978.
• Seveau S, Keller H, Maxfield FR, et al. (2000). "Neutrophil polarity and locomotion are associated with surface redistribution of leukosialin (CD43), an antiadhesive membrane molecule". Blood. 95 (8): 2462–70. doi:10.1182/blood.V95.8.2462. PMID 10753822.
• Fratazzi C, Manjunath N, Arbeit RD, et al. (2000). "A macrophage invasion mechanism for mycobacteria implicating the extracellular domain of CD43". J. Exp. Med. 192 (2): 183–92. doi:10.1084/jem.192.2.183. PMC 2193255. PMID 10899905.
• Santana MA, Pedraza-Alva G, Olivares-Zavaleta N, et al. (2000). "CD43-mediated signals induce DNA binding activity of AP-1, NF-AT, and NFkappa B transcription factors in human T lymphocytes". J. Biol. Chem. 275 (40): 31460–8. doi:10.1074/jbc.M005231200. PMID 10908570.
• van den Berg TK, Nath D, Ziltener HJ, et al. (2001). "Cutting edge: CD43 functions as a T cell counterreceptor for the macrophage adhesion receptor sialoadhesin (Siglec-1)". J. Immunol. 166 (6): 3637–40. doi:10.4049/jimmunol.166.6.3637. PMID 11238599.

External links

• CD43+antigen at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
• Human SPN genome location and SPN gene details page in the UCSC Genome Browser.