QPNC-PAGE
Biochemistry technique for separating proteins / From Wikipedia, the free encyclopedia
QPNC-PAGE, or Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis, is a bioanalytical, one-dimensional, high-resolution and high-precision electrophoresis technique applied in biochemistry and bioinorganic chemistry to separate proteins quantitatively by isoelectric point and by continuous elution from a gel column.[1]
This hybrid variant of native analytical gel electrophoresis and preparative polyacrylamide gel electrophoresis is used by biologists to resolve macromolecules in solution with high recovery, for example, into active or native metalloproteins in biological samples or into properly and improperly folded metal cofactor-containing proteins or into protein isoforms in complex protein mixtures.[2]