Bicinchoninic acid
Chemical compound / From Wikipedia, the free encyclopedia
Bicinchoninic acid /baɪsɪnkɔːnɪnɪk/ is a weak acid composed of two carboxylated quinoline rings.
Names | |
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Preferred IUPAC name
[2,2′-Biquinoline]-4,4′-dicarboxylic acid | |
Other names
Bicinchoninic acid 4,4′-Dicarboxy-2,2′-biquinoline 2,2'-Biquinoline-4,4'-dicarboxylic acid | |
Identifiers | |
3D model (JSmol) |
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ChEMBL | |
ChemSpider |
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ECHA InfoCard | 100.013.628 |
PubChem CID |
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UNII | |
CompTox Dashboard (EPA) |
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Properties | |
C20H12N2O4 | |
Molar mass | 344.326 g·mol−1 |
Appearance | Cream colored powder |
Odor | Characteristic odor[vague] |
Melting point | 352 °C (666 °F; 625 K) decomposes |
Partially soluble in cold water, hot water[vague] | |
Hazards | |
Occupational safety and health (OHS/OSH): | |
Main hazards |
Will irritate eyes and mucous membranes. |
NFPA 704 (fire diamond) | |
Safety data sheet (SDS) | External MSDS |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Bicinchoninic is an organic compound with the formula (C9H5NCO2H)2. The molecule consists of a pair of quinoline rings, each bearing a carboxylic acid group. Its sodium salt forms a purple complex with cuprous ions.[1][2][3]
Bicinchoninic acid is most commonly employed in the bicinchoninic acid (BCA) assay, which is used to determine the total level of protein in a solution. Bicinchoninic acid is used to detect the presence of cuprous ions, due to its purple coloration via a biuret reaction. In this assay, two molecules of bicinchoninic acid chelate a single Cu+ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.[4]