亚硝酸正丁酯可以用亚硝酸(由亞硝酸鹽和無機酸原位製備)和正丁醇製備而成。 亚硝酸正丁酯高度可燃。 亚硝酸正丁酯是一種會用作情慾芳香劑的吸入性藥物(英语:inhalant),會帶來短暫的欣快感。 亚硝酸正丁酯也可以製備其他化學試劑,例如铜铁试剂就是由苯胲、亚硝酸正丁酯和氨來製備。 C. S. Marvel. "Cupferron
containing levmetamfetamine identified in 341.20(b)(1) when used in an inhalant dosage form. The product delivers in each 800 milliliters of air 0.04 to
Edwards RW, Oetting ER. Inhalant use in the United States. Kozel N, Sloboda Z, De La Rosa M (编). Epidemiology of Inhalant Abuse: An International Perspective