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丙酮酸激酶(英語:Pyruvate kinase,EC 2.7.1.40)是糖酵解过程中的一类酶,催化磷酸基团从磷酸烯醇式丙酮酸(PEP)转移到ADP的反应,生成一分子丙酮酸和一分子ATP.
丙酮酸激酶所催化的反应为
反应需要镁离子作为辅因子。
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