西斯汀禮拜堂（粵拼：sai1 si1 ting1 lai5 baai3 tong4，拉丁文：Sacellum Sixtinum；意大利文：Cappella Sistina）係一座喺梵蒂岡宗座宮殿入面嘅天主教堂，喺聖伯多祿大教堂隔離，因為米開朗基羅畫嘅《創世紀》穹頂畫，同埋壁畫《末日審判》而出名。
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