核酸检测目前以PCR相关技术为主,过程比较容易开发,是被广泛用于检测患者呼吸道病毒和COVID-19确诊的黄金方法(英语:Gold standard (test)),但其缺点是一般对实验室清潔及医疗防护等技术条件要求高、依现有操作获得可靠结果大多比较费时; 恒温核酸扩增技术(英语:VariantsofPCR
Development of a real-time PCR assay for the diagnosis of scrub typhus cases in India and evidence of the prevalence of new genotype of O. tsutsugamushi
Cabral, J. A.; Bastos, E. Molecular sexing of birds: A comparative review of polymerase chain reaction (PCR)-based methods. Theriogenology. 2012, 78 (4):