組織蛋白酶G

組織蛋白酶G（英文：Cathepsin G）是一種在人體中由CTSG基因編碼的蛋白質。它是儲存在嗜天青顆粒細胞中的糜蛋白酶家族的三種絲氨酸蛋白酶之一，也是肽酶S1蛋白家族的成員。組織蛋白酶G在消除細胞內病原體和分解炎症部位的組織以及抗炎反應中起重要作用。^[5][6][7][8]

組織蛋白酶G
已知的結構 PDB 直系同源搜索: PDBe RCSB PDBID列表 1AU8、​1CGH、​1KYN、​1T32
識別號
別名	CTSG；, CATG, CG, cathepsin G
外部ID	OMIM：116830 MGI：88563 HomoloGene：105646 GeneCards：CTSG
基因位置（人類） 染色體 14號染色體[1] 基因座 14q12 起始 24,573,518 bp[1] 終止 24,576,250 bp[1]
基因位置（小鼠） 染色體 小鼠14號染色體[2] 基因座 14 C3|14 28.19 cM 起始 56,337,338 bp[2] 終止 56,340,031 bp[2]
RNA表達模式
查閱更多表達數據
基因本體 分子功能 • heparin binding • 肽酶活性 • 血漿蛋白結合 • 水解酶活性 • serine-type endopeptidase activity • serine-type peptidase activity 細胞組分 • 細胞外間質 • 細胞膜 • secretory granule • cell surface • 外排體 • 細胞核 • 細胞外空間 • azurophil granule lumen • 應激顆粒 • 細胞外區域 • 細胞質 • collagen-containing extracellular matrix 生物學過程 • extracellular matrix disassembly • 蛋白酶解 • angiotensin maturation • protein phosphorylation • response to lipopolysaccharide • positive regulation of immune response • defense response to bacterium • 免疫反應 • defense response to Gram-positive bacterium • antimicrobial humoral response • neutrophil degranulation • defense response to fungus • neutrophil-mediated killing of gram-positive bacterium Sources:Amigo / QuickGO
直系同源
物種	人類	小鼠
Entrez	1511	13035
Ensembl	ENSG00000100448	ENSMUSG00000040314
UniProt	P08311	P28293
mRNA​序列	​NM_001911	NM_007800
蛋白序列	NP_001902	NP_031826
基因位置​（UCSC）	Chr 14: 24.57 – 24.58 Mb	Chr 14: 56.34 – 56.34 Mb
PubMed​查找	[3]	[4]
維基數據
檢視/編輯人類 檢視/編輯小鼠

結構

基因

CTSG基因位於14號染色體q11.2，由5個外顯子組成。催化三聯體的每個殘基都位於一個單獨的外顯子上。通過掃描整個編碼區已鑑定出五個基因多態性。^[9]組織蛋白酶G是通過基因複製從共同祖先進化而來的同源蛋白酶之一。^[10]

蛋白質

組織蛋白酶G是一種255-氨基酸殘基的蛋白質，包括一個18-殘基信號肽、一個N端的兩個殘基活化肽和一個C端延伸。^[11]組織蛋白酶G的活性依賴於由天冬氨酸、組氨酸和絲氨酸殘基組成的催化三聯體，這些殘基在一級序列中廣泛分離，但在三級結構中酶的活性位點彼此靠近。^[12]

作用

組織蛋白酶G具有與胰凝乳蛋白酶C（英語：Chymotrypsin C）相似的特異性，但它與其他免疫絲氨酸蛋白酶如中性粒細胞彈性蛋白酶（英語：Neutrophil elastase）和顆粒酶的關係最為密切。^[13]作為一種中性粒細胞絲氨酸蛋白酶，它首先被鑑定為降解酶，它在細胞內作用以降解攝入的宿主病原體，並在細胞外作用於炎症部位的細胞外基質成分的分解。^[14]它定位於中性粒細胞胞外陷阱，通過其對DNA的高親和力，這是絲氨酸蛋白酶的不尋常特性。^[13]該基因存在利用替代多腺苷酸化信號的轉錄變體。^[15]還發現組織蛋白酶G對革蘭氏陰性菌和革蘭氏陽性菌具有廣譜抗菌作用，與上述功能無關。^[16]組織蛋白酶G的其他功能已發現，包括切割受體、將血管緊張素I轉化為血管緊張素II、血小板激活和誘導氣道黏膜下腺分泌.^{[17][18][19][20][21]}還發現了該酶在 血腦屏障 破壞中的潛在影響。^[22]

臨床意義

發現組織蛋白酶G在多種疾病中發揮重要作用，包括類風濕性關節炎、冠狀動脈疾病、牙周炎、缺血再灌注損傷（英語：Reperfusion injury）和骨轉移。^{[23][24][25][26][27]}它還與多種感染性炎症疾病有關，包括慢性阻塞性肺病、急性呼吸窘迫綜合症和囊腫性纖維化。^[28][29][30]最近的一項研究表明，具有CTSG基因多態性的患者患慢性術後疼痛的風險更高，這表明組織蛋白酶G可能作為疼痛控制的新靶點和預測慢性術後疼痛的潛在標誌物。^[31]在圓錐角膜的研究中報告了組織蛋白酶G的上調。^[32]

交互作用

已發現組織蛋白酶G與以下物質相互作用：

• SERPINB1（英語：SERPINB1）^[33]

組織蛋白酶G受以下因素抑制：

• [2-[3-[[(1-苯甲酰基-4-哌啶基)甲氨基]羰基]-2-萘基]-1-(1-萘基)-2-氧代乙基]-膦酸(KPA)^[34]
• Paubrasilia彈性蛋白酶抑制劑^[35]
• N-芳酰基O-磺化氨基糖苷類^[36]

組織蛋白酶G降低以下水平：

• 低密度脂蛋白^[37]

參見

• 組織蛋白酶

參考文獻

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2. GRCm38: Ensembl release 89: ENSMUSG00000040314 - Ensembl, May 2017
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4. Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
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拓展閱讀

• Shafer WM, Katzif S, Bowers S, Fallon M, Hubalek M, Reed MS, Veprek P, Pohl J. Tailoring an antibacterial peptide of human lysosomal cathepsin G to enhance its broad-spectrum action against antibiotic-resistant bacterial pathogens. Current Pharmaceutical Design. 2002, 8 (9): 695–702. PMID 11945165. doi:10.2174/1381612023395376.
• Cohen AB, Stevens MD, Miller EJ, Atkinson MA, Mullenbach G. Generation of the neutrophil-activating peptide-2 by cathepsin G and cathepsin G-treated human platelets. The American Journal of Physiology. August 1992, 263 (2 Pt 1): L249–56. PMID 1387511. doi:10.1152/ajplung.1992.263.2.L249.
• Sasaki T, Ueno-Matsuda E. Immunocytochemical localization of cathepsins B and G in odontoclasts of human deciduous teeth. Journal of Dental Research. December 1992, 71 (12): 1881–4. PMID 1452887. S2CID 27658837. doi:10.1177/00220345920710120501.
• Maison CM, Villiers CL, Colomb MG. Proteolysis of C3 on U937 cell plasma membranes. Purification of cathepsin G. Journal of Immunology. August 1991, 147 (3): 921–6. PMID 1861080.
• Brandt E, Van Damme J, Flad HD. Neutrophils can generate their activator neutrophil-activating peptide 2 by proteolytic cleavage of platelet-derived connective tissue-activating peptide III. Cytokine. July 1991, 3 (4): 311–21. PMID 1873479. doi:10.1016/1043-4666(91)90499-4.
• Kargi HA, Campbell EJ, Kuhn C. Elastase and cathepsin G of human monocytes: heterogeneity and subcellular localization to peroxidase-positive granules. The Journal of Histochemistry and Cytochemistry. August 1990, 38 (8): 1179–86. PMID 2164060. doi:10.1177/38.8.2164060 .
• Pratt CW, Tobin RB, Church FC. Interaction of heparin cofactor II with neutrophil elastase and cathepsin G. The Journal of Biological Chemistry. April 1990, 265 (11): 6092–7. PMID 2318847. doi:10.1016/S0021-9258(19)39296-8 .
• Gabay JE, Scott RW, Campanelli D, Griffith J, Wilde C, Marra MN, Seeger M, Nathan CF. Antibiotic proteins of human polymorphonuclear leukocytes. Proceedings of the National Academy of Sciences of the United States of America. July 1989, 86 (14): 5610–4. Bibcode:1989PNAS...86.5610G. PMC 297672 . PMID 2501794. doi:10.1073/pnas.86.14.5610 .
• Hohn PA, Popescu NC, Hanson RD, Salvesen G, Ley TJ. Genomic organization and chromosomal localization of the human cathepsin G gene. The Journal of Biological Chemistry. August 1989, 264 (23): 13412–9. PMID 2569462. doi:10.1016/S0021-9258(18)80012-6 .
• Livesey SA, Buescher ES, Krannig GL, Harrison DS, Linner JG, Chiovetti R. Human neutrophil granule heterogeneity: immunolocalization studies using cryofixed, dried and embedded specimens. Scanning Microscopy. Supplement. 1989, 3: 231–9; discussion 239–40. PMID 2616953.
• Campbell EJ, Silverman EK, Campbell MA. Elastase and cathepsin G of human monocytes. Quantification of cellular content, release in response to stimuli, and heterogeneity in elastase-mediated proteolytic activity. Journal of Immunology. November 1989, 143 (9): 2961–8. PMID 2681419.
• Salvesen G, Farley D, Shuman J, Przybyla A, Reilly C, Travis J. Molecular cloning of human cathepsin G: structural similarity to mast cell and cytotoxic T lymphocyte proteinases. Biochemistry. April 1987, 26 (8): 2289–93. PMID 3304423. doi:10.1021/bi00382a032.
• Heck LW, Rostand KS, Hunter FA, Bhown A. Isolation, characterization, and amino-terminal amino acid sequence analysis of human neutrophil cathepsin G from normal donors. Analytical Biochemistry. October 1986, 158 (1): 217–27. PMID 3799965. doi:10.1016/0003-2697(86)90612-3.
• Crocker J, Jenkins R, Burnett D. Immunohistochemical localization of cathepsin G in human tissues. The American Journal of Surgical Pathology. May 1985, 9 (5): 338–43. PMID 3911778. S2CID 23124253. doi:10.1097/00000478-198505000-00003.
• Klickstein LB, Kaempfer CE, Wintroub BU. The granulocyte-angiotensin system. Angiotensin I-converting activity of cathepsin G. The Journal of Biological Chemistry. December 1982, 257 (24): 15042–6. PMID 6294088. doi:10.1016/S0021-9258(18)33390-8 .
• LaRosa CA, Rohrer MJ, Benoit SE, Barnard MR, Michelson AD. Neutrophil cathepsin G modulates the platelet surface expression of the glycoprotein (GP) Ib-IX complex by proteolysis of the von Willebrand factor binding site on GPIb alpha and by a cytoskeletal-mediated redistribution of the remainder of the complex. Blood. July 1994, 84 (1): 158–68. PMID 7517206. doi:10.1182/blood.V84.1.158.158 .
• Owen CA, Campbell MA, Sannes PL, Boukedes SS, Campbell EJ. Cell surface-bound elastase and cathepsin G on human neutrophils: a novel, non-oxidative mechanism by which neutrophils focus and preserve catalytic activity of serine proteinases. The Journal of Cell Biology. November 1995, 131 (3): 775–89. PMC 2120617 . PMID 7593196. doi:10.1083/jcb.131.3.775.
• Savage MJ, Iqbal M, Loh T, Trusko SP, Scott R, Siman R. Cathepsin G: localization in human cerebral cortex and generation of amyloidogenic fragments from the beta-amyloid precursor protein. Neuroscience. June 1994, 60 (3): 607–19. PMID 7936190. S2CID 24998185. doi:10.1016/0306-4522(94)90490-1.
• Grisolano JL, Sclar GM, Ley TJ. Early myeloid cell-specific expression of the human cathepsin G gene in transgenic mice. Proceedings of the National Academy of Sciences of the United States of America. September 1994, 91 (19): 8989–93. Bibcode:1994PNAS...91.8989G. PMC 44732 . PMID 8090757. doi:10.1073/pnas.91.19.8989 .
• Maruyama K, Sugano S. Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene. January 1994, 138 (1–2): 171–4. PMID 8125298. doi:10.1016/0378-1119(94)90802-8.

外部連結

• The MEROPS online database for peptidases and their inhibitors: S01.133 （頁面存檔備份，存於互聯網檔案館）

組織蛋白酶G引用了美國國家醫學圖書館提供的資料，這些資料屬於公共領域。