C-5甾醇去飽和酶(英語:C-5 sterol desaturase,或稱為甾醇C-5去飽和酶 sterol C-5 desaturase,縮寫C5SD)是廣泛存在於真核生物體內,參與類固醇生成的酶,依賴NADH將類固醇底物的5、6號碳原子上的氫脫去,生成帶碳碳雙鍵的產物。在人體中,C-5去飽和酶將7-膽甾烯醇脫氫生成7-脫氫膽固醇,最後轉化為膽固醇。在真菌釀酒酵母(Saccharomyces cerevisiae)中,C5SD催化表甾醇脫氫生成5-脫氫表甾醇,最後轉化為麥角固醇,在一些植物中,C5SD參與油菜素類固醇、谷固醇的合成[1]。
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