玻璃化是植物幼苗在生長過程中出現的一種類似水浸的半透明化的生理畸形現象[1]。在組織培養過程中發生玻璃化會導致幼苗發育不良、分化能力低下以及難以成活等症狀[1]。玻璃化現象最早是在1964年進行石竹(Dianthus caryophyllus)莖尖組織培養時發現的[2]。1981年才提出了「玻璃化」(Hyperhydricity)一詞[3]。
Zhao, Qingping; Liang, Yuping; Zhou, Fangyuan; Zhao, Xiang. Research Progress of Hyperhydricity Mechanism in Plant Seedling Growth. Chinese Bulletin of Botany. 2022-01-01, 57 (1): 90. doi:10.11983/CBB21126.
Phillips, Douglas J.; Matthews, Gary J. Growth and Development of Carnation Shoot Tips In vitro. Botanical Gazette. 1964-03, 125 (1): 7–12. doi:10.1086/336237.
Debergh, P.; Harbaoui, Y.; Lemeur, R. Mass propagation of globe artichoke (Cynara scolymus): Evaluation of different hypotheses to overcome vitrification with special reference to water potential. Physiologia Plantarum. 1981-10, 53 (2): 181–187. doi:10.1111/j.1399-3054.1981.tb04130.x.