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分枝桿菌目（學名：Mycobacteriales）為放線菌綱的一目細菌。此目細菌的模式種為分枝桿菌屬(Mycobacterium)。
本科包括以下屬：
- 棒狀桿菌科 Corynebacteriaceae Lehmann and Neumann 1907
- 迪茨氏菌科 Dietziaceae Rainey et al. 1997
- 戈登氏菌科 Gordoniaceae Rainey et al. 1997
- Lawsonellaceae Nouioui et al. 2018
- 分枝桿菌科 Mycobacteriaceae Chester 1897
- 諾卡氏菌科 Nocardiaceae Castellani and Chalmers 1919
- 慢反應脂肪酸菌科 Segniliparaceae Butler et al. 2005
- Speluncibacteraceae Lee et al. 2024
- Speluncibacter Lee et al. 2024
- Speluncibacter jeojiensis Lee et al. 2024[1]
- Speluncibacter Lee et al. 2024
- 束村氏菌科 Tsukamurellaceae Rainey et al. 1997
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