雙脫氧核苷酸(英語:Dideoxynucleotide)是DNA聚合酶的鏈終止性抑制劑,應用於桑格DNA測序法。這些核苷酸亦稱2',3'-雙脫氧核苷酸,常簡寫為ddNTPs(ddGTP、ddATP、ddTTP與ddCTP)。

2',3'-雙脫氧腺苷三磷酸(ddATP)的分子結構

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