TSB 使用胰蛋白脢水解後的酪和黃豆提供氨基酸和其他含氮營養物質。葡萄糖為TSB的主要能量來源。除此之外，該培養基亦含有氯化鈉保持滲透壓的平衡，磷酸氫二鉀作為緩衝物質以保持pH值。TSA為TSB加入瓊脂作為凝固劑配置而成。
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