金胺O

金胺O是一种二芳基甲烷萤光染料，常态下为黄色针状结晶。极易溶于水，易溶于乙醇。

Auramine O
IUPAC名 bis[4-(dimethylamino)phenyl]methaniminium chloride
别名	Basic yellow 2, Pyocatanium aureum, aizen auramine, Pyoktanin Yellow, Canary Yellow, Pyoktanin, or C.I. 41000
识别
CAS号	2465-27-2  Y
PubChem	17170
ChemSpider	16254
SMILES	[N@H]=C(c1ccc(N(C)C)cc1)c2ccc(N(C)C)cc2
InChI	1/C17H21N3/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4/h5-12,18H,1-4H3
InChIKey	JPIYZTWMUGTEHX-UHFFFAOYAY
性质
化学式	C17H22ClN3
摩尔质量	303.83 g·mol−1
熔点	267 °C
危险性
警示术语	R：R22 R24 R40
安全术语	S：S36/37 S45
若非注明，所有数据均出自标准状态（25 ℃，100 kPa）下。

金胺O可用于抗酸细菌（例如Mycobacterium）, 其方法类似抗酸染色法。^[1] 也被当做希夫试剂的萤光版来使用。^[2]

在金胺-若丹明染色法中与若丹明B一起用来给结核杆菌染色。^[3][4] 同时也可用作防腐剂。

参考文献

1. Kommareddi S, Abramowsky C, Swinehart G, Hrabak L. Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis. Hum Pathol. 1984, 15 (11): 1085–9. PMID 6208117. doi:10.1016/S0046-8177(84)80253-1.
2. Khavkin T, Kudryavtseva M, Dragunskaya E, Polotsky Y, Kudryavtsev B. Fluorescent PAS-reaction study of the epithelium of normal rabbit ileum and after challenge with enterotoxigenic Escherichia coli. Gastroenterology. 1980, 78 (4): 782–90. PMID 6986320.
3. Truant J, Brett W, Thomas W. Fluorescence microscopy of tubercle bacilli stained with auramine and rhodamine. Henry Ford Hosp Med Bull. 1962, 10: 287–96. PMID 13922644.
4. Arrowood M, Sterling C. Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection. J Clin Microbiol. 1989, 27 (7): 1490–5. PMC 267601 . PMID 2475523.

外部链接

• Auramine O spectra data （页面存档备份，存于互联网档案馆）