烯醇化酶(英语:Enolase,又被称为磷酸烯醇式丙酮酸水合酶,phosphopyruvate hydratase)是负责催化糖酵解的第九步反应,2-磷酸甘油酸(2-PG)转化为磷酸烯醇式丙酮酸(PEP)的金属酶。其催化的化学反应式为:
- 2-磷酸-D-甘油酸 磷酸烯醇式丙酮酸 + H2O
烯醇化酶裂合酶家族中的水裂解酶类,用于裂解碳氧键。
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