16号美国国道（英语：U.S. Route 16）是一条东西方向的美国国道。该公路连接了南达科他州拉皮德城和怀俄明州黄石国家公园，全长540英里。
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| 管理方 | Wyoming Department of Transportation[*]、South Dakota Department of Transportation[*] |
| 启用时间 | 1926 |
| 公路系统 | |
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