磷酸二酯键（英语：phosphodiester bond）也称为“3′,5′-磷酸二酯键”或“磷酸双酯键”，是核酸分子中的磷酸基团的磷原子与另外两个五碳糖分子的碳原子之间形成的共价键。这种形式的键结于DNA及RNA分子中负责将分别位于两个核糖上的3号碳与5号碳连结起来。[1]
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