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双脱氧核苷酸(英语:Dideoxynucleotide)是DNA聚合酶的链终止性抑制剂,应用于桑格DNA测序法。这些核苷酸亦称2',3'-双脱氧核苷酸,常简写为ddNTPs(ddGTP、ddATP、ddTTP与ddCTP)。

2',3'-双脱氧腺苷三磷酸(ddATP)的分子结构

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