Dihidrofolat reduktaza

Dihidrofolat reduktaza (EC 1.5.1.3, tetrahidrofolatna dehidrogenaza, DHFR, pteridinska reduktaza:dihidrofolat reduktaza, dihidrofolatna reduktaza:timidilat sintaza, timidilatna sintetaza-dihidrofolat reduktaza, folna kiselina reduktaza, folna reduktaza, dihidrofolna kiselina reduktaza, dihidrofolna reduktaza, 7,8-dihidrofolat reduktaza, NADPH-dihidrofolat reduktaza) je enzim sa sistematskim imenom 5,6,7,8-tetrahidrofolat:NADP⁺ oksidoreduktaza.^[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju

5,6,7,8-tetrahidrofolat + NADP⁺ ${\displaystyle \rightleftharpoons }$ 7,8-dihidrofolat + NADPH + H⁺

Dihidrofolat reduktaza
Identifikatori
EC broj	1.5.1.3
CAS broj	2594015
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Ovaj enzim iz životinaj i nekih mikroorganizama takođe u manjoj meri redukuje folat do 5,6,7,8-tetrahidrofolata.

Reference

1. Blakley, R.L. and MacDougall, B.M. (1961). „Dihydrofolic reductase from Streptococcus faecalis R”. J. Biol. Chem. 236: 1163-1163.
2. Bolin, J.T., Filman, D.J., Matthews, D.A. and Kraut, J. (1982). „Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 Å resolution. I. General features and binding of methotrexate”. J. Biol. Chem. 257: 13650-13662. PMID 6815178.
3. Kaufman, B.T. and Gardiner, R.C. (1966). „Studies on dihydrofolic reductase. I. Purification and properties of dihydrofolic reductase from chicken liver”. J. Biol. Chem. 241: 1319-1328. PMID 4379915.
4. Young, I.G. and Gibson, F. (1969). „Regulation of the enzymes involved in the biosynthesis of 2,3-dihydroxybenzoic acid in Aerobacter aerogenes and Escherichia coli”. Biochim. Biophys. Acta 177: 401-411. PMID 4306838.

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.

Spoljašnje veze

• MeSH Dihydrofolate+reductase