デアセトキシセファロスポリンCヒドロキシラーゼ（deacetoxycephalosporin-C hydroxylase）は、次の化学反応を触媒する酸化還元酵素である。
- デアセトキシセファロスポリンC + 2-オキソグルタル酸 + O2 デアセチルセファロスポリンC + コハク酸 + CO2
| デアセトキシセファロスポリンCヒドロキシラーゼ | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| 識別子 | |||||||||
| EC番号 | 1.14.11.26 | ||||||||
| データベース | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB構造 | RCSB PDB PDBj PDBe PDBsum | ||||||||
| |||||||||
反応式の通り、この酵素の基質はデアセトキシセファロスポリンCと2-オキソグルタル酸とO2、生成物はデアセチルセファロスポリンCとコハク酸とCO2である。
組織名はdeacetoxycephalosporin-C,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)で、別名にdeacetylcephalosporin C synthase、3'-methylcephem hydroxylase、DACS、DAOC hydroxylase、deacetoxycephalosporin C hydroxylaseがある。
参考文献
- Dotzlaf, J.E. and Yeh, W.K. (1987). “Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium.”. J. Bacteriol. 169: 1611-1618. PMID 3558321.
- Baker, B.J., Dotzlaf, J.E. and Yeh, W.K. (1991). “Deacetoxycephalosporin C hydroxylase of Streptomyces clavuligerus. Purification, characterization, bifunctionality, and evolutionary implication.”. J. Biol. Chem. 266: 5087-5093. PMID 2002049.
- Coque, J.J., Enguita, F.J., Cardoza, R.E., Martin, J.F. and Liras, P. (1996). “Characterization of the cefF gene of Nocardia lactamdurans encoding a 3'-methylcephem hydroxylase different from the 7-cephem hydroxylase.”. Appl. Microbiol. Biotechnol. 44: 605-609. PMID 8703431.
- Ghag, S.K., Brems, D.N., Hassell, T.C. and Yeh, W.K. (1996). “Refolding and purification of Cephalosporium acremonium deacetoxycephalosporin C synthetase/hydroxylase from granules of recombinant Escherichia coli.”. Appl. Biochem. 24: 109-119. PMID 8865604.
- Lloyd, M.D., Lipscomb, S.J., Hewitson, K.S., Hensgens, C.M., Baldwin, J.E. and Schofield, C.J. (2004). “Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase.”. J. Biol. Chem. 279: 15420-15426. PMID 14734549.
- Wu, X.B., Fan, K.Q., Wang, Q.H. and Yang, K.Q. (2005). “C-terminus mutations of Acremonium chrysogenum deacetoxy/deacetylcephalosporin C synthase with improved activity toward penicillin analogs.”. FEMS Microbiol. Lett. 246: 103-110. PMID 15869968.
- Martín, J.F., Gutiérrez, S., Fernández, F.J., Velasco, J., Fierro, F., Marcos, A.T. and Kosalkova, K. (1994). “Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins.”. Antonie Van Leeuwenhoek 65: 227-43. PMID 7847890.
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