Thới Lai is a new rural district (huyện) of Cần Thơ City in the Mekong Delta region of Vietnam. As of 2009 the district had a population of 120,964, rising to 147,546 in 2018. The district covers an area of 255.66 km2. The district capital lies at Thới Lai. The district was formed in 2009 from the southern portion of Cờ Đỏ district.