Roxbury Presbyterian Church is a historic Presbyterian church at 328 Warren Street in Boston, Massachusetts, United States. The Gothic church building was designed by John C. Spofford and built in 1891 for a congregation organized in 1881. Built of Roxbury pudding stone, it has asymmetrical massing partitioned into polygonal sections. The tower at the northwest corner starts on a square base, rising with differently-shaped sections to a pyramidal roof. Half of the building's original construction cost was paid for by the Gilchrist brothers, owners of the locally prominent Gilchrist Department Store.