The Supermarine Spitfire is a British single-seat fighter aircraft used in the Second World War.
Spitfire may also refer to:
Road
- Triumph Spitfire, a small two-seat British sports car from Triumph Motor Company developed in the 1960s
- BSA Spitfire, a British motorcycle launched in 1966
- Spitfire (American band), a metalcore band from Virginia Beach, Virginia
- Spitfire (English band), a garage rock band from Crawley, West Sussex, England
- Spitfire (Russian band), a ska band from Saint Petersburg, Russia
- The Spitfire Band, a Canadian big band
- Spitfire (Jefferson Starship album), 1976
- Spitfire (EP), a 2011 EP by Porter Robinson
- "Spitfire", the EP's title track
- Spitfire (LeAnn Rimes album), 2013
- "Spitfire" (song), a 2004 single by the British electronic dance music group The Prodigy
- "Spitfire", a track by Public Service Broadcasting from their 2012 EP The War Room and 2013 album Inform - Educate - Entertain
- "Spitfire", a 2017 single by the Israeli musical duo Infected Mushroom
- "Spitfire", a 2008 single by the Italian Eurobeat duo Go 2, featured in the anime MF Ghost
- Spitfire Audio, creators of digital sample libraries
- Spitfire Records, a heavy metal-oriented record label
- Spitfire (BBS), a software program for operating a Bulletin Board System
- Spitfire sawfly, a type of insect and its caterpillar found in Australia
- Kent Spitfires, the name adopted by Kent County Cricket Club when playing One Day or Twenty20 matches
- London Spitfire, an esports team in the Overwatch League
- Windsor Spitfires, a junior ice hockey team in the Ontario Hockey League
- Eastleigh F.C., a non-league football club in Hampshire, England, nicknamed "The Spitfires"