60S ribosomal protein L7

60S ribosomal protein L7 is a protein that in humans is encoded by the RPL7 gene.^[5][6][7]

RPL7
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4UG0, 4V6X, 5AJ0
Identifiers
Aliases	RPL7, L7, humL7-1, ribosomal protein L7, ribosomal protein uL30
External IDs	OMIM: 604166; MGI: 98073; HomoloGene: 87772; GeneCards: RPL7; OMA:RPL7 - orthologs
Gene location (Human) Chr. Chromosome 8 (human)[1] Band 8q21.11 Start 73,290,242 bp[1] End 73,295,789 bp[1]
Gene location (Mouse) Chr. Chromosome 1 (mouse)[2] Band 1|1 A3 Start 16,171,519 bp[2] End 16,174,886 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ganglionic eminence Achilles tendon ventricular zone left ovary gastric mucosa right ovary canal of the cervix monocyte smooth muscle tissue body of uterus Top expressed in epiblast ventricular zone ganglionic eminence blastocyst uterus zone of skin lens morula esophagus thymus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding protein homodimerization activity DNA binding mRNA binding RNA binding structural constituent of ribosome Cellular component ribosome nucleolus cytoplasm cytosol nucleus membrane focal adhesion extracellular exosome polysome cytosolic large ribosomal subunit postsynaptic density ribonucleoprotein complex Biological process SRP-dependent cotranslational protein targeting to membrane maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) viral transcription nuclear-transcribed mRNA catabolic process, nonsense-mediated decay cytoplasmic translation ribosomal large subunit biogenesis translational initiation protein biosynthesis rRNA processing Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 6129 19989 Ensembl ENSG00000147604 ENSMUSG00000043716 UniProt P18124 P14148 RefSeq (mRNA) NM_000971 NM_001363737 NM_011291 RefSeq (protein) NP_000962 NP_001350666 NP_035421 Location (UCSC) Chr 8: 73.29 – 73.3 Mb Chr 1: 16.17 – 16.17 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Function

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus sub-motif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.^[7]

Interactions

RPL7 has been shown to interact with ZNF7.^[8] It interacts with HIV-1 Gag protein through Zinc Finger of HIV-1 Gag.^[9]

References

1. GRCh38: Ensembl release 89: ENSG00000147604 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000043716 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Seshadri T, Uzman JA, Oshima J, Campisi J (September 1993). "Identification of a transcript that is down-regulated in senescent human fibroblasts. Cloning, sequence analysis, and regulation of the human L7 ribosomal protein gene". The Journal of Biological Chemistry. 268 (25): 18474–80. doi:10.1016/S0021-9258(17)46650-6. PMID 8360149.
6. Hemmerich P, von Mikecz A, Neumann F, Sözeri O, Wolff-Vorbeck G, Zoebelein R, Krawinkel U (January 1993). "Structural and functional properties of ribosomal protein L7 from humans and rodents". Nucleic Acids Research. 21 (2): 223–31. doi:10.1093/nar/21.2.223. PMC 309096. PMID 8441630.
7. "Entrez Gene: RPL7 ribosomal protein L7".
8. Witte S, Krawinkel U (August 1997). "Specific interactions of the autoantigen L7 with multi-zinc finger protein ZNF7 and ribosomal protein S7". The Journal of Biological Chemistry. 272 (35): 22243–7. doi:10.1074/jbc.272.35.22243. PMID 9268371.
9. Mekdad HE, Boutant E, Karnib H, Biedma ME, Sharma KK, Malytska I, et al. (August 2016). "Characterization of the interaction between the HIV-1 Gag structural polyprotein and the cellular ribosomal protein L7 and its implication in viral nucleic acid remodeling". Retrovirology. 13 (1): 54. doi:10.1186/s12977-016-0287-4. PMC 4982112. PMID 27515235.

Further reading

• Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal proteins". Biochemistry and Cell Biology. 73 (11–12): 933–47. doi:10.1139/o95-101. PMID 8722009.
• von Mikecz A, Hemmerich P, Peter HH, Krawinkel U (December 1994). "Characterization of eukaryotic protein L7 as a novel autoantigen which frequently elicits an immune response in patients suffering from systemic autoimmune disease". Immunobiology. 192 (1–2): 137–54. doi:10.1016/S0171-2985(11)80413-4. PMID 7750987.
• Neumann F, Hemmerich P, von Mikecz A, Peter HH, Krawinkel U (January 1995). "Human ribosomal protein L7 inhibits cell-free translation in reticulocyte lysates and affects the expression of nuclear proteins upon stable transfection into Jurkat T-lymphoma cells". Nucleic Acids Research. 23 (2): 195–202. doi:10.1093/nar/23.2.195. PMC 306654. PMID 7862521.
• Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Witte S, Krawinkel U (August 1997). "Specific interactions of the autoantigen L7 with multi-zinc finger protein ZNF7 and ribosomal protein S7". The Journal of Biological Chemistry. 272 (35): 22243–7. doi:10.1074/jbc.272.35.22243. PMID 9268371.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (May 1998). "A map of 75 human ribosomal protein genes". Genome Research. 8 (5): 509–23. doi:10.1101/gr.8.5.509. PMID 9582194.
• Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI (January 2002). "Directed proteomic analysis of the human nucleolus". Current Biology. 12 (1): 1–11. Bibcode:2002CBio...12....1A. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. S2CID 14132033.
• Brezillon S, Lannoy V, Franssen JD, Le Poul E, Dupriez V, Lucchetti J, Detheux M, Parmentier M (January 2003). "Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8". The Journal of Biological Chemistry. 278 (2): 776–83. doi:10.1074/jbc.M206396200. PMID 12401809.
• Kasai H, Nadano D, Hidaka E, Higuchi K, Kawakubo M, Sato TA, Nakayama J (May 2003). "Differential expression of ribosomal proteins in human normal and neoplastic colorectum". The Journal of Histochemistry and Cytochemistry. 51 (5): 567–74. doi:10.1177/002215540305100502. PMID 12704204. S2CID 25865715.
• Odintsova TI, Müller EC, Ivanov AV, Egorov TA, Bienert R, Vladimirov SN, Kostka S, Otto A, Wittmann-Liebold B, Karpova GG (April 2003). "Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing". Journal of Protein Chemistry. 22 (3): 249–58. doi:10.1023/A:1025068419698. PMID 12962325. S2CID 10710245.
• Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ (January 2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nature Biotechnology. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (January 2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
• Ko JR, Wu JY, Kirby R, Li IF, Lin A (July 2006). "Mapping the essential structures of human ribosomal protein L7 for nuclear entry, ribosome assembly and function". FEBS Letters. 580 (16): 3804–10. doi:10.1016/j.febslet.2006.05.073. PMID 16797011. S2CID 25168455.
• Wu WC, Liu HW, Lin A (February 2007). "Human ribosomal protein L7 displays an ER binding property and is involved in ribosome-ER association". FEBS Letters. 581 (4): 651–7. doi:10.1016/j.febslet.2007.01.023. PMID 17258209. S2CID 30461645.