60S ribosomal protein L12

60S ribosomal protein L12 is a protein that in humans is encoded by the RPL12 gene.^[5][6]

RPL12
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4V6X, 5AJ0, 3J92, 4V5Z, 5A8L
Identifiers
Aliases	RPL12, L12, ribosomal protein L12
External IDs	OMIM: 180475; MGI: 98002; HomoloGene: 128047; GeneCards: RPL12; OMA:RPL12 - orthologs
Gene location (Human) Chr. Chromosome 9 (human)[1] Band 9q33.3 Start 127,447,674 bp[1] End 127,451,406 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2|2 B Start 32,851,571 bp[2] End 32,855,357 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Achilles tendon olfactory zone of nasal mucosa uterine tube left ovary canal of the cervix skin of abdomen skin of leg right ovary lactiferous gland gallbladder Top expressed in epiblast embryo embryo yolk sac esophagus ileum ventricular zone uterus morula lens More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding structural constituent of ribosome rRNA binding RNA binding large ribosomal subunit rRNA binding Cellular component cytosol extracellular exosome membrane cytosolic large ribosomal subunit focal adhesion ribosome extracellular matrix nucleus nucleolus cytoplasm postsynaptic density large ribosomal subunit Biological process translational initiation SRP-dependent cotranslational protein targeting to membrane nuclear-transcribed mRNA catabolic process, nonsense-mediated decay ribosomal large subunit assembly viral transcription protein biosynthesis rRNA processing Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 6136 269261 Ensembl ENSG00000197958 ENSMUSG00000038900 UniProt P30050 P35979 RefSeq (mRNA) NM_000976 NM_009076 RefSeq (protein) NP_000967 NP_033102 Location (UCSC) Chr 9: 127.45 – 127.45 Mb Chr 2: 32.85 – 32.86 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Function

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. The protein binds directly to the 26S rRNA. This gene is co-transcribed with the U65 snoRNA, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.^[6]

Interactions

RPL12 has been shown to interact with CDC5L.^[7]

References

1. GRCh38: Ensembl release 89: ENSG00000197958 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000038900 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Chu W, Presky DH, Swerlick RA, Burns DK (March 1993). "The primary structure of human ribosomal protein L12". Nucleic Acids Res. 21 (3): 749. doi:10.1093/nar/21.3.749. PMC 309184. PMID 8441690.
6. "Entrez Gene: RPL12 ribosomal protein L12".
7. Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (December 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". EMBO J. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC 305846. PMID 11101529.

Further reading

• Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal proteins". Biochem. Cell Biol. 73 (11–12): 933–47. doi:10.1139/o95-101. PMID 8722009.
• Kato S, Sekine S, Oh SW, Kim NS, Umezawa Y, Abe N, Yokoyama-Kobayashi M, Aoki T (1995). "Construction of a human full-length cDNA bank". Gene. 150 (2): 243–50. doi:10.1016/0378-1119(94)90433-2. PMID 7821789.
• Uchiumi T, Kominami R (1997). "Binding of mammalian ribosomal protein complex P0.P1.P2 and protein L12 to the GTPase-associated domain of 28 S ribosomal RNA and effect on the accessibility to anti-28 S RNA autoantibody". J. Biol. Chem. 272 (6): 3302–8. doi:10.1074/jbc.272.6.3302. PMID 9013569.
• Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (1998). "A map of 75 human ribosomal protein genes". Genome Res. 8 (5): 509–23. doi:10.1101/gr.8.5.509. PMID 9582194.
• Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (2001). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". EMBO J. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC 305846. PMID 11101529.
• Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. Bibcode:2002CBio...12....1A. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. S2CID 14132033.
• Plafker SM, Macara IG (2002). "Ribosomal Protein L12 Uses a Distinct Nuclear Import Pathway Mediated by Importin 11". Mol. Cell. Biol. 22 (4): 1266–75. doi:10.1128/MCB.22.4.1266-1275.2002. PMC 134630. PMID 11809816.
• Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
• Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243. S2CID 14294292.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.

External links

• Overview of all the structural information available in the PDB for UniProt: P30050 (Human 60S ribosomal protein L12) at the PDBe-KB.