RAD51L3

DNA repair protein RAD51 homolog 4 is a protein that in humans is encoded by the RAD51L3 gene.^[5][6]

RAD51D
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2KZ3
Identifiers
Aliases	RAD51D, BROVCA4, R51H3, RAD51L3, TRAD, RAD51 paralog D
External IDs	OMIM: 602954; MGI: 1261809; HomoloGene: 2156; GeneCards: RAD51D; OMA:RAD51D - orthologs
Gene location (Human) Chr. Chromosome 17 (human)[1] Band 17q12 Start 35,092,221 bp[1] End 35,121,522 bp[1]
Gene location (Mouse) Chr. Chromosome 11 (mouse)[2] Band 11 C|11 50.3 cM Start 82,767,260 bp[2] End 82,781,440 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sperm oocyte right testis left testis stromal cell of endometrium ganglionic eminence prefrontal cortex epithelium of colon right frontal lobe granulocyte Top expressed in granulocyte right kidney proximal tubule zygote secondary oocyte primary oocyte Rostral migratory stream embryo dentate gyrus of hippocampal formation granule cell mesenteric lymph nodes More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding nucleotide binding ATP-dependent activity, acting on DNA DNA strand exchange activity single-stranded DNA binding protein binding four-way junction DNA binding gamma-tubulin binding double-stranded DNA binding endodeoxyribonuclease activity ATP binding Cellular component cytoplasm centrosome replication fork chromosome microtubule organizing center telomere Rad51B-Rad51C-Rad51D-XRCC2 complex cytoskeleton nucleus nucleoplasm Biological process strand invasion response to ionizing radiation nucleotide-excision repair reciprocal meiotic recombination DNA recombination interstrand cross-link repair chromosome organization regulation of cell cycle cellular response to DNA damage stimulus mitotic recombination telomere maintenance DNA repair meiotic DNA recombinase assembly double-strand break repair via homologous recombination Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5892 19364 Ensembl ENSG00000185379 ENSMUSG00000018841 UniProt O75771 O55230 RefSeq (mRNA) NM_001142571 NM_002878 NM_133627 NM_133628 NM_133629 NM_133630 NM_001277938 NM_001277939 NM_001277941 NM_001277942 NM_011235 RefSeq (protein) NP_001136043 NP_002869 NP_598332 NP_001264867 NP_001264868 NP_001264870 NP_001264871 NP_035365 Location (UCSC) Chr 17: 35.09 – 35.12 Mb Chr 11: 82.77 – 82.78 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinant DNA repair. Several alternatively spliced transcript variants of this gene have been described, but the biological validity of some of them has not been determined.^[6]

Interactions

RAD51L3 has been shown to interact with:

• Bloom syndrome protein,^[7]
• RAD51C,^[8][9] and
• XRCC2.^{[7][8][10][11]}

References

1. GRCh38: Ensembl release 89: ENSG00000185379 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000018841 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Pittman DL, Weinberg LR, Schimenti JC (June 1998). "Identification, characterization, and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene". Genomics. 49 (1): 103–11. doi:10.1006/geno.1998.5226. PMID 9570954.
6. "Entrez Gene: RAD51L3 RAD51-like 3 (S. cerevisiae)".
7. Braybrooke JP, Li JL, Wu L, Caple F, Benson FE, Hickson ID (November 2003). "Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D)". J. Biol. Chem. 278 (48): 48357–66. doi:10.1074/jbc.M308838200. hdl:10026.1/10297. PMID 12975363.
8. Liu N, Schild D, Thelen MP, Thompson LH (February 2002). "Involvement of Rad51C in two distinct protein complexes of Rad51 paralogs in human cells". Nucleic Acids Res. 30 (4): 1009–15. doi:10.1093/nar/30.4.1009. PMC 100342. PMID 11842113.
9. Miller KA, Yoshikawa DM, McConnell IR, Clark R, Schild D, Albala JS (March 2002). "RAD51C interacts with RAD51B and is central to a larger protein complex in vivo exclusive of RAD51". J. Biol. Chem. 277 (10): 8406–11. doi:10.1074/jbc.M108306200. PMID 11744692.
10. Schild D, Lio YC, Collins DW, Tsomondo T, Chen DJ (June 2000). "Evidence for simultaneous protein interactions between human Rad51 paralogs". J. Biol. Chem. 275 (22): 16443–9. doi:10.1074/jbc.M001473200. PMID 10749867.
11. Hussain S, Wilson JB, Medhurst AL, Hejna J, Witt E, Ananth S, Davies A, Masson JY, Moses R, West SC, de Winter JP, Ashworth A, Jones NJ, Mathew CG (June 2004). "Direct interaction of FANCD2 with BRCA2 in DNA damage response pathways". Hum. Mol. Genet. 13 (12): 1241–8. doi:10.1093/hmg/ddh135. PMID 15115758.

Further reading

• Cartwright R, Dunn AM, Simpson PJ, Tambini CE, Thacker J (1998). "Isolation of novel human and mouse genes of the recA/RAD51 recombination-repair gene family". Nucleic Acids Res. 26 (7): 1653–9. doi:10.1093/nar/26.7.1653. PMC 147465. PMID 9512535.
• Kawabata M, Saeki K (1999). "Multiple alternative transcripts of the human homologue of the mouse TRAD/R51H3/RAD51D gene, a member of the rec A/RAD51 gene family". Biochem. Biophys. Res. Commun. 257 (1): 156–62. doi:10.1006/bbrc.1999.0413. PMID 10092526.
• Schild D, Lio YC, Collins DW, Tsomondo T, Chen DJ (2000). "Evidence for simultaneous protein interactions between human Rad51 paralogs". J. Biol. Chem. 275 (22): 16443–9. doi:10.1074/jbc.M001473200. PMID 10749867.
• Braybrooke JP, Spink KG, Thacker J, Hickson ID (2000). "The RAD51 family member, RAD51L3, is a DNA-stimulated ATPase that forms a complex with XRCC2". J. Biol. Chem. 275 (37): 29100–6. doi:10.1074/jbc.M002075200. PMID 10871607.
• Miller KA, Yoshikawa DM, McConnell IR, Clark R, Schild D, Albala JS (2002). "RAD51C interacts with RAD51B and is central to a larger protein complex in vivo exclusive of RAD51". J. Biol. Chem. 277 (10): 8406–11. doi:10.1074/jbc.M108306200. PMID 11744692.
• Masson JY, Tarsounas MC, Stasiak AZ, Stasiak A, Shah R, McIlwraith MJ, Benson FE, West SC (2001). "Identification and purification of two distinct complexes containing the five RAD51 paralogs". Genes Dev. 15 (24): 3296–307. doi:10.1101/gad.947001. PMC 312846. PMID 11751635.
• Sigurdsson S, Van Komen S, Bussen W, Schild D, Albala JS, Sung P (2001). "Mediator function of the human Rad51B-Rad51C complex in Rad51/RPA-catalyzed DNA strand exchange". Genes Dev. 15 (24): 3308–18. doi:10.1101/gad.935501. PMC 312844. PMID 11751636.
• Kurumizaka H, Ikawa S, Nakada M, Enomoto R, Kagawa W, Kinebuchi T, Yamazoe M, Yokoyama S, Shibata T (2002). "Homologous pairing and ring and filament structure formation activities of the human Xrcc2*Rad51D complex". J. Biol. Chem. 277 (16): 14315–20. doi:10.1074/jbc.M105719200. PMID 11834724.
• Wiese C, Collins DW, Albala JS, Thompson LH, Kronenberg A, Schild D (2002). "Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cells". Nucleic Acids Res. 30 (4): 1001–8. doi:10.1093/nar/30.4.1001. PMC 100332. PMID 11842112.
• Liu N, Schild D, Thelen MP, Thompson LH (2002). "Involvement of Rad51C in two distinct protein complexes of Rad51 paralogs in human cells". Nucleic Acids Res. 30 (4): 1009–15. doi:10.1093/nar/30.4.1009. PMC 100342. PMID 11842113.
• Godthelp BC, Artwert F, Joenje H, Zdzienicka MZ (2002). "Impaired DNA damage-induced nuclear Rad51 foci formation uniquely characterizes Fanconi anemia group D1". Oncogene. 21 (32): 5002–5. doi:10.1038/sj.onc.1205656. PMID 12118380.
• Braybrooke JP, Li JL, Wu L, Caple F, Benson FE, Hickson ID (2003). "Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D)". J. Biol. Chem. 278 (48): 48357–66. doi:10.1074/jbc.M308838200. hdl:10026.1/10297. PMID 12975363.
• Hillman RT, Green RE, Brenner SE (2004). "An unappreciated role for RNA surveillance". Genome Biol. 5 (2): R8. doi:10.1186/gb-2004-5-2-r8. PMC 395752. PMID 14759258.
• Tarsounas M, Davies AA, West SC (2004). "RAD51 localization and activation following DNA damage". Philos. Trans. R. Soc. Lond. B Biol. Sci. 359 (1441): 87–93. doi:10.1098/rstb.2003.1368. PMC 1693300. PMID 15065660.
• Tarsounas M, Muñoz P, Claas A, Smiraldo PG, Pittman DL, Blasco MA, West SC (2004). "Telomere maintenance requires the RAD51D recombination/repair protein". Cell. 117 (3): 337–47. doi:10.1016/S0092-8674(04)00337-X. PMID 15109494. S2CID 16478454.
• Hussain S, Wilson JB, Medhurst AL, Hejna J, Witt E, Ananth S, Davies A, Masson JY, Moses R, West SC, de Winter JP, Ashworth A, Jones NJ, Mathew CG (2004). "Direct interaction of FANCD2 with BRCA2 in DNA damage response pathways". Hum. Mol. Genet. 13 (12): 1241–8. doi:10.1093/hmg/ddh135. PMID 15115758.
• Rodríguez-López R, Osorio A, Ribas G, Pollán M, Sánchez-Pulido L, de la Hoya M, Ruibal A, Zamora P, Arias JI, Salazar R, Vega A, Martínez JI, Esteban-Cardeñosa E, Alonso C, Letón R, Urioste Azcorra M, Miner C, Armengod ME, Carracedo A, González-Sarmiento R, Caldés T, Díez O, Benítez J (2004). "The variant E233G of the RAD51D gene could be a low-penetrance allele in high-risk breast cancer families without BRCA1/2 mutations". Int. J. Cancer. 110 (6): 845–9. doi:10.1002/ijc.20169. PMID 15170666. S2CID 6309119.
• Loveday C, Turnbull C, Ramsay E, Hughes D, Ruark E, Frankum JR, Bowden G, Kalmyrzaev B, Warren-Perry M, Snape K, Adlard JW, Barwell J, Berg J, Brady AF, Brewer C, Brice G, Chapman C, Cook J, Davidson R, Donaldson A, Douglas F, Greenhalgh L, Henderson A, Izatt L, Kumar A, Lalloo F, Miedzybrodzka Z, Morrison PJ, Paterson J, Porteous M, Rogers MT, Shanley S, Walker L, Eccles D, Evans DG, Renwick A, Seal S, Lord CJ, Ashworth A, Reis-Filho JS, Antoniou AC, Rahman N (2011-08-07). "Germline mutations in RAD51D confer susceptibility to ovarian cancer". Nat. Genet. 43 (9): 879–82. doi:10.1038/ng.893. PMC 4845885. PMID 21822267.