Potosí or Potosi may refer to:
- Potosi Mountain (Nevada), U.S.
- Potosí mountain range or Cordillera de Potosí, to the southeast of the city of Potosí, Bolivia
- Potosi Peak, in the Sneffels Range, Colorado, US
- Cerro de Potosí, a mountain near the city of Potosí, Bolivia, also known as Cerro Rico; a Spanish colonial mining site
- Cerro Potosí, a mountain in Nuevo León, Mexico
- Huayna Potosí, a mountain in the La Paz Department, Bolivia
- Wayna Potosí (Oruro), a mountain in the Oruro Department, Bolivia
- Potosi (barque), an 1895 German sailing ship named after the Bolivian city
- Potosi Correctional Center, a prison near Potosi, Missouri
- Potosi Mining District, in Clark County, Nevada
- Potosi, Trelawny, Jamaica, a former sugar plantation
- Potosi Pinyon (Pinus culminicola), a species of pine tree native to northeast Mexico
- Potosi pupfish (Cyprinodon alvarezi), a species of fish once endemic to Mexico, but now extinct in the wild