DNA polymerase alpha subunit 2

DNA polymerase alpha subunit 2 is an enzyme that in humans is encoded by the POLA2 gene.^[5][6][7]

POLA2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2KEB, 4E2I, 4Y97, 5EXR
Identifiers
Aliases	POLA2, DNA polymerase alpha 2, accessory subunit
External IDs	MGI: 99690; HomoloGene: 48121; GeneCards: POLA2; OMA:POLA2 - orthologs
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11q13.1 Start 65,261,920 bp[1] End 65,305,589 bp[1]
Gene location (Mouse) Chr. Chromosome 19 (mouse)[2] Band 19|19 A Start 5,990,570 bp[2] End 6,014,230 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ventricular zone embryo gonad ganglionic eminence mucosa of transverse colon apex of heart bone marrow cells spleen canal of the cervix granulocyte Top expressed in tail of embryo genital tubercle epiblast spermatocyte yolk sac somite pharynx maxillary prominence mandibular prominence thymus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding DNA-directed DNA polymerase activity protein heterodimerization activity molecular function Cellular component alpha DNA polymerase:primase complex nucleus nucleoplasm cytosol Biological process DNA replication DNA biosynthetic process DNA replication initiation telomere maintenance via semi-conservative replication G1/S transition of mitotic cell cycle Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 23649 18969 Ensembl ENSG00000014138 ENSMUSG00000024833 UniProt Q14181 P33611 RefSeq (mRNA) NM_002689 NM_001164057 NM_008893 RefSeq (protein) NP_002680 NP_001157529 NP_032919 Location (UCSC) Chr 11: 65.26 – 65.31 Mb Chr 19: 5.99 – 6.01 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Interactions

POLA2 has been shown to interact with PARP1.^[8]

See also

• DNA Polymerase
• DNA polymerase alpha

References

1. GRCh38: Ensembl release 89: ENSG00000014138 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000024833 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Collins KL, Russo AA, Tseng BY, Kelly TJ (Dec 1993). "The role of the 70 kDa subunit of human DNA polymerase alpha in DNA replication". EMBO J. 12 (12): 4555–66. doi:10.1002/j.1460-2075.1993.tb06144.x. PMC 413886. PMID 8223465.
6. Huang D, Jokela M, Tuusa J, Skog S, Poikonen K, Syväoja JE (Jul 2001). "E2F mediates induction of the Sp1-controlled promoter of the human DNA polymerase ɛ B-subunit gene POLE2". Nucleic Acids Res. 29 (13): 2810–21. doi:10.1093/nar/29.13.2810. PMC 55767. PMID 11433027.
7. "Entrez Gene: POLA2 polymerase (DNA directed), alpha 2 (70kD subunit)".
8. Dantzer F, Nasheuer HP, Vonesch JL, de Murcia G, Ménissier-de Murcia J (Apr 1998). "Functional association of poly(ADP-ribose) polymerase with DNA polymerase alpha-primase complex: a link between DNA strand break detection and DNA replication". Nucleic Acids Res. 26 (8): 1891–8. doi:10.1093/nar/26.8.1891. PMC 147507. PMID 9518481.

Further reading

• Pollok S, Stoepel J, Bauerschmidt C, Kremmer E, Nasheuer HP (2003). "Regulation of eukaryotic DNA replication at the initiation step". Biochem. Soc. Trans. 31 (Pt 1): 266–9. doi:10.1042/BST0310266. PMID 12546699.
• Nasheuer HP, Moore A, Wahl AF, Wang TS (1991). "Cell cycle-dependent phosphorylation of human DNA polymerase alpha". J. Biol. Chem. 266 (12): 7893–903. doi:10.1016/S0021-9258(20)89534-9. PMID 1902230.
• Dantzer F, Nasheuer HP, Vonesch JL, de Murcia G, Ménissier-de Murcia J (1998). "Functional association of poly(ADP-ribose) polymerase with DNA polymerase alpha-primase complex: a link between DNA strand break detection and DNA replication". Nucleic Acids Res. 26 (8): 1891–8. doi:10.1093/nar/26.8.1891. PMC 147507. PMID 9518481.
• Schneider A, Smith RW, Kautz AR, Weisshart K, Grosse F, Nasheuer HP (1998). "Primase activity of human DNA polymerase alpha-primase. Divalent cations stabilize the enzyme activity of the p48 subunit". J. Biol. Chem. 273 (34): 21608–15. doi:10.1074/jbc.273.34.21608. PMID 9705292.
• Taneja P, Gu J, Peng R, Carrick R, Uchiumi F, Ott RD, Gustafson E, Podust VN, Fanning E (2002). "A dominant-negative mutant of human DNA helicase B blocks the onset of chromosomal DNA replication". J. Biol. Chem. 277 (43): 40853–61. doi:10.1074/jbc.M208067200. PMID 12181327.
• Lehner B, Sanderson CM (2004). "A Protein Interaction Framework for Human mRNA Degradation". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
• Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243. S2CID 14294292.

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human DNA polymerase alpha subunit B