Mephisto or Mephistopheles is one of the chief demons of German literary tradition.
Mephisto or Mephistopheles may also refer to:
- Méphisto, a 1931 French film
- Mephisto (1981 film), a German-Hungarian film based on Klaus Mann's novel Mephisto
- Mephistopheles, an antagonist in the film Ghost Rider
- Dr. Mephesto, a mad scientist in the South Park TV series
- Mephistopheles (Xena), a villain in the Xena: Warrior Princess TV series
- Mephisto, a character on the Australian television series, Double the Fist
- Mephisto, a character in the anime Suite PreCure
- Mephisto Pheles, a son of Satan in the manga/anime Blue Exorcist
- Mephisto, a character in the feature art film The Last Faust
- Mephisto, a 1912 British silent film written by Leedham Bantock and directed by Alfred de Manby and F. Martin Thornton
- Captain Mephisto, a time-travelling, shapeshifting pirate in the 1945 Republic Pictures movie serial Manhunt of Mystery Island
- Dark Mephisto and Dark Mephisto (Zwei), two of the villains in Ultraman Nexus
- Mephisto, one of the main villains in the series Lolirock
- Mephisto (automaton), a chess-playing pseudo-automaton
- Mephisto (chess computer), a line of chess computers sold by Hegener & Glaser
- Mephistopheles (Dungeons & Dragons), a character in Dungeons & Dragons
- Mr. Mephisto, an early video game
- Mephisto, a character in the Diablo series
- Mephisto (crossword), a long-running competition in the Sunday Times, set by various compilers
- Mephistopheles, a character in Limbus Company
- Mephisto, a character in Arknights
- Mephiles the Dark, the main antagonist of the 2006 video game Sonic the Hedgehog
- Mephisto (comics), a supervillain in the Marvel Comics universe
- Mephisto (novel), a novel by Klaus Mann
- Mefisto, a novel by John Banville
- Mephisto, a Japanese literary magazine which publishes the winner of the Mephisto Prize
- Mephisto or Mephi, a freedom-fighting organization in the 1920 novel We by Yevgeny Zamyatin
- Mefisto (band), a Swedish black metal band
- Mephisto Waltzes (1859–1885), waltzes by Franz Liszt
- Mefistofele (1868), an opera by Italian composer Arrigo Boito
- "Mephisto" (song), a 2023 song by Queen Bee, served as an ending theme to the anime adaptation of Oshi no Ko
- "Mephisto", a song by Moonspell from the 1996 album Irreligious
- "Mephisto", a song by Klaus Schulze from the 2005 album Moonlake
- Mephisto, a character in the two-part rock opera by Kamelot, Epica and The Black Halo
- "Mephistopheles", a song by Deicide from the album Deicide
- "Mephisto", a song by Bushido from the 2018 album Mythos; see Bushido discography
- Mr. Mistoffelees, a black cat in T.S. Eliot's Old Possum's Book of Practical Cats and in the musical Cats