Manzanillo or manzanillo (Spanish for "little apple", literally) may refer to:
- Manzanillo, Colima, a city in Mexico
- Manzanillo, Cuba, a city in the province of Granma
- Manzanillo Port, a port in the province of Monte Cristi, Dominican Republic
- Manzanillo, Valladolid, a municipality in the province of Valladolid, Spain
- Manzanillo Bay, a bay on the Atlantic coast of Panama, near the eastern entrance to the Panama Canal
- Manzanillo, Limón, a fishing village in the south-east of Costa Rica, on the Caribbean Sea coast
- Manzanillo (olive) (Olea europaea 'Manzanillo', also known as the "olive of Seville") the most common variety of Spanish olive, a medium-sized green to purple-black olive cultivar grown especially in and around Seville, Andalusia; also, the Manzanillo olive tree as a whole, rather than just its fruit; often misspelled Manzanilla
- Manzanillo (sternwheeler), a steamboat in the U.S. state of Oregon in the late 19th century
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