Leaf area index

Leaf area index (LAI) is a dimensionless quantity that characterizes plant canopies. It is defined as the one-sided green leaf area per unit ground surface area (LAI = leaf area / ground area, m² / m²) in broadleaf canopies.^[1] In conifers, three definitions for LAI have been used:

• Half of the total needle surface area per unit ground surface area ^[2]
• Projected (or one-sided, in accordance the definition for broadleaf canopies) needle area per unit ground area
• Total needle surface area per unit ground area ^[3]

The definition “half the total leaf area” pertains to biological processes, such as gas exchange, whereas the definition “projected leaf area” was disregarded because the projection of a given area in one direction may differ in another direction when leaves are not flat, thick, or 3D-shaped. Moreover, “ground surface area” is specifically defined as “horizontal ground surface area” to clarify LAI on a sloping surface. The definition “half the total leaf area per unit horizontal ground surface area” is suitable for all kinds of leaves and flat or sloping surfaces.^[4]

A leaf area index (LAI) expresses the leaf area per unit ground or trunk surface area of a plant and is commonly used as an indicator of the growth rate of a plant. LAI is a complex variable that relates not only to the size of the canopy, but also to its density, and the angle at which leaves are oriented in relation to one another and to light sources. In addition, LAI varies with seasonal changes in plant activity,^[5] and is typically highest in the spring when new leaves are being produced and lowest in late summer or early fall when leaves senesce (and may be shed). The study of LAI is called "phyllometry."^[6]

Interpretation and application

LAI is a measure for the total area of leaves per unit ground area and directly related to the amount of light that can be intercepted by plants. It is an important variable used to predict photosynthetic primary production, evapotranspiration and as a reference tool for crop growth. As such, LAI plays an essential role in theoretical production ecology. An inverse exponential relation between LAI and light interception, which is linearly proportional to the primary production rate, has been established:^{[citation needed][7][8]}

${\displaystyle P=P_{\max }\left(1-e^{-c\cdot LAI}\right)}$

where P_max designates the maximum primary production and ${\displaystyle c}$ designates a crop-specific growth coefficient. This inverse exponential function is called the primary production function.

LAI ranges from 0 (bare ground) to over 10 (dense conifer forests).^[9]

Determining LAI

LAI can be determined directly by taking a statistically significant sample of foliage from a plant canopy, measuring the leaf area per sample plot and dividing it by the plot land surface area. Indirect methods measure canopy geometry or light extinction and relate it to LAI.^[10]

Direct methods

Direct methods can be easily applied on deciduous species by collecting leaves during leaf fall in traps of certain area distributed below the canopy. The area of the collected leaves can be measured using a leaf area meter or an image scanner and image analysis software (ImageJ) and mobile applications (Leafscan, Petiole Pro, Easy Leaf Area). The measured leaf area can then be divided by the area of the traps to obtain LAI. Alternatively, leaf area can be measured on a sub-sample of the collected leaves and linked to the leaf dry mass (e.g. via Specific Leaf Area, SLA cm²/g). That way it is not necessary to measure the area of all leaves one by one, but weigh the collected leaves after drying (at 60–80 °C for 48 h). Leaf dry mass multiplied by the specific leaf area is converted into leaf area.
Direct methods in evergreen species are necessarily destructive. However, they are widely used in crops and pastures by harvesting the vegetation and measuring leaf area within a certain ground surface area. It is very difficult (and also unethical) to apply such destructive techniques in natural ecosystems, particularly in forests of evergreen tree species. Foresters have developed techniques that determine leaf area in evergreen forests through allometric relationships.
Due to the difficulties and the limitations of the direct methods for estimating LAI, they are mostly used as reference for indirect methods that are easier and faster to apply.

Indirect methods

A hemispherical photograph of forest canopy. The ratio of the area of canopy to sky is used to approximate LAI.

Indirect methods of estimating LAI in situ can be divided roughly into at least three categories:

1. indirect contact LAI measurements such as plumb lines and inclined point quadrats^{[citation needed]}
2. indirect non-contact measurements
3. indirect estimation from remote sensing such as lidar^[11] and multispectral imaging^[12]

Due to the subjectivity and labor involved with the first method, indirect non-contact measurements are typically preferred. Non-contact LAI tools, such as hemispherical photography, Hemiview Plant Canopy Analyser from Delta-T Devices, the CI-110 Plant Canopy Analyzer from CID Bio-Science, LAI-2200 Plant Canopy Analyzer from LI-COR Biosciences and the LP-80 LAI ceptometer from Decagon Devices, measure LAI in a non-destructive way. Hemispherical photography methods estimate LAI and other canopy structure attributes from analyzing upward-looking fisheye photographs taken beneath the plant canopy. The LAI-2200 calculates LAI and other canopy structure attributes from solar radiation measurements made with a wide-angle optical sensor. Measurements made above and below the canopy are used to determine canopy light interception at five angles, from which LAI is computed using a model of radiative transfer in vegetative canopies based on Beer's law . The LP-80 calculates LAI by means of measuring the difference between light levels above the canopy and at ground level, and factoring in the leaf angle distribution, solar zenith angle, and plant extinction coefficient. Such indirect methods, where LAI is calculated based upon observations of other variables (canopy geometry, light interception, leaf length and width,^[13] etc.) are generally faster, amenable to automation, and thereby allow for a larger number of spatial samples to be obtained. For reasons of convenience when compared to the direct (destructive) methods, these tools are becoming more and more important.

Disadvantages of methods

The disadvantage of the direct method is that it is destructive, time consuming and expensive, especially if the study area is very large.

The disadvantage of the indirect method is that in some cases it can underestimate the value of LAI in very dense canopies, as it does not account for leaves that lie on each other, and essentially act as one leaf according to the theoretical LAI models.^[14][15] Ignorance of non-randomness within canopies may cause underestimation of LAI up to 25%, introducing path length distribution in the indirect method can improve the measuring accuracy of LAI.^[16] Indirect estimation of LAI is also sensitive to the data analysis methods of choices.^[17]

See also

• Canopy (ecology)
• Hemispherical photography
• Normalized Difference Vegetation Index
• Specific leaf area
• Plant growth analysis

References

1. Watson, D.J. (1947). "Comparative physiological studies on the growth of field crops: I. Variation in net assimilation rate and leaf area between species and varieties and within and between years". Annals of Botany. 11: 41–76. doi:10.1093/oxfordjournals.aob.a083148.
2. Chen, J.M.; Black, T.A. (1992). "Defining leaf area index for non-flat leaves". Agricultural and Forest Meteorology. 57: 1–12. doi:10.1016/0168-1923(91)90074-z.
3. GHOLZ, HENRY L.; FITZ, FRANKLIN K.; WARING, R.H. (1976). "Leaf area differences associated with old-growth forest communities in the western Oregon Cascades". Canadian Journal of Forest Research. 6 (1): 49–57. doi:10.1139/x76-007. S2CID 85319218.
4. Yan, G.J.; Hu, R.H.; Luo, J.H.; Marie, W.; Jiang, H.L.; Mu, X.H.; Xie, D.H.; Zhang, W.M. (2019). "Review of indirect optical measurements of leaf area index: Recent advances, challenges, and perspectives". Agricultural and Forest Meteorology. 265: 390–411. Bibcode:2019AgFM..265..390Y. doi:10.1016/j.agrformet.2018.11.033.
5. Maass, JoséManuel; Vose, James M.; Swank, Wayne T.; Martínez-Yrízar, Angelina (1995-06-01). "Seasonal changes of leaf area index (LAI) in a tropical deciduous forest in west Mexico". Forest Ecology and Management. 74 (1): 171–180. Bibcode:1995ForEM..74..171M. doi:10.1016/0378-1127(94)03485-F. ISSN 0378-1127.
6. Tomažič, Irma; Korošec-Koruza, Zora (2003-11-01). "Validity of Phyllometric Parameters Used to Differentiate Locan Vitis Vinifera L. Cultivars". Genetic Resources and Crop Evolution. 50 (7): 773–778. doi:10.1023/A:1025085012808. ISSN 1573-5109. S2CID 6333777.
7. Firman, D. M., and E. J. Allen. “Relationship between Light Interception, Ground Cover and Leaf Area Index in Potatoes.” The Journal of Agricultural Science 113, no. 3 (December 1989): 355–59. doi:10.1017/S0021859600070040. https://www.niab.com/uploads/files/Light_interception_ground_cover_LAI_Firman_Allen_1989.pdf
8. Asner, Gregory P, Jonathan M O Scurlock, and Jeffrey A Hicke. “Global Synthesis of Leaf Area Index Observations: Implications for Ecological and Remote Sensing Studies.” Global Ecology, 2003, 15. http://www2.geog.ucl.ac.uk/~mdisney/teaching/teachingNEW/GMES/LAI_GLOBAL_RS.pdf
9. Iio, Atsuhiro; Hikosaka, Kouki; Anten, Niels P. R.; Nakagawa, Yoshiaki; Ito, Akihiko (2014). "Global dependence of field-observed leaf area index in woody species on climate: a systematic review". Global Ecology and Biogeography. 23 (3): 274–285. Bibcode:2014GloEB..23..274I. doi:10.1111/geb.12133. ISSN 1466-8238.
10. Breda, N (2003). "Ground-based measurements of leaf area index: A review of methods, instruments and current controversies". Journal of Experimental Botany. 54 (392): 2403–2417. doi:10.1093/jxb/erg263. PMID 14565947.
11. Zhao, Kaiguang; Popescu, Sorin (2009). "Lidar-based mapping of leaf area index and its use for validating GLOBCARBON satellite LAI product in a temperate forest of the southern USA". Remote Sensing of Environment. 113 (8): 1628–1645. Bibcode:2009RSEnv.113.1628Z. doi:10.1016/j.rse.2009.03.006.
12. Chen, JM; Cihlar, Josef (1996). "Retrieving leaf area index of boreal conifer forests using Landsat TM images". Remote Sensing of Environment. 55 (2): s 153–162. Bibcode:1996RSEnv..55..153C. doi:10.1016/0034-4257(95)00195-6.
13. Blanco, F.F.; Folegatti, M.V. (2003). "A new method for estimating the leaf area index of cucumber and tomato plants". Horticultura Brasileira. 21 (4): 666–669. doi:10.1590/S0102-05362003000400019.
14. Wilhelm, W.W.; Ruwe, K.; Schlemmer, M.R. (2000). "Comparisons of three Leaf Area Index Meters in a Corn Canopy". Crop Science. 40 (4): 1179–1183. doi:10.2135/cropsci2000.4041179x. S2CID 6461200.
15. Zhao, Kaiguang; García, Mariano; Guo, Qinghua; Liu, Shu; Chen, Gang; Zhang, Xuesong; Zhou, Yuyu; Xuelian, Meng. (2015). "Terrestrial lidar remote sensing of forests: Maximum likelihood estimates of canopy profile, leaf area index, and leaf angle distribution". Agricultural and Forest Meteorology. 209: 100–113. Bibcode:2015AgFM..209..100Z. doi:10.1016/j.agrformet.2015.03.008.
16. Hu, Ronghai; Yan, Guangjian; Mu, Xihan; Luo, Jinghui (2014). "Indirect measurement of leaf area index on the basis of path length distribution". Remote Sensing of Environment. 155: 239–247. Bibcode:2014RSEnv.155..239H. doi:10.1016/j.rse.2014.08.032.
17. Zhao, Kaiguang; Ryu, Youngryel; Hu, Tongxi; Garcia, Mariano; Yang, Li; Liu, Zhen; Londo, Alexis; Wang, Chao (2019). "How to better estimate leaf area index and leaf angle distribution from digital hemispherical photography? Switching to a binary nonlinear regression paradigm". Methods in Ecology and Evolution. 10 (11): 1864–1874. Bibcode:2019MEcEv..10.1864Z. doi:10.1111/2041-210X.13273.

Notes

• Non-Existence of an Optimum Leaf Area Index for the Production Rate of White Clover Grown Under Constant Conditions
• Law, B.E., T. Arkebauer, J.L. Campbell, J. Chen, O. Sun, M. Schwartz, C. van Ingen, S. Verma. 2008. Terrestrial Carbon Observations: Protocols for Vegetation Sampling and Data Submission. Report 55, Global Terrestrial Observing System. FAO, Rome. 87 pp.
• LAI Definition of University of Giessen, Germany