John Seymour may refer to:
- John Hayward (MP for Bridport) (c. 1355–1407), alias Seymour, member of parliament for Bridport, U.K.
- John Seymour (MP for Bristol) (fl. 1351), member of parliament for Bristol, U.K.
- John Seymour (1425–1463), British landowner and member of parliament
- John Seymour (died 1464), British knight and member of parliament
- John Seymour (died 1491) (1450s–1491), member of the British landed gentry and grandfather of Queen Jane Seymour
- Sir John Seymour (1474–1536), father of Queen Jane Seymour, third wife of Henry VIII of England
- John Seymour (died 1567), member of parliament for Great Bedwyn, U.K.
- John Seymour (died 1552), member of parliament for Wootton Bassett, U.K.
- John Seymour (died 1618), member of parliament for Great Bedwyn, U.K.
- John Seymour, 4th Duke of Somerset (before 1646–1675), British peer and member of parliament
- John Seymour (Maryland governor) (1649–1709), royal governor of the Maryland colony in the Americas, then part of England
- John Seymour (Gloucestershire MP) (died 1663), member of parliament for Gloucestershire
- John Seymour (California politician) (born 1937), senator from California, U.S.
- John Seymour, 19th Duke of Somerset (born 1952), British landowner and elected hereditary peer
- John Webb Seymour (1777–1819), English aristocrat and amateur geologist