3beta-hydroxy-5beta-steroid dehydrogenase

In enzymology, a 3beta-hydroxy-5beta-steroid dehydrogenase (EC 1.1.1.277) is an enzyme that catalyzes the chemical reaction

3beta-hydroxy-5beta-pregnane-20-one + NADP⁺ ${\displaystyle \rightleftharpoons }$ 5beta-pregnan-3,20-dione + NADPH + H⁺

3beta-hydroxy-5beta-steroid dehydrogenase
Identifiers
EC no.	1.1.1.277
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Thus, the two substrates of this enzyme are 3beta-hydroxy-5beta-pregnane-20-one and NADP⁺, whereas its 3 products are 5beta-pregnan-3,20-dione, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is 3beta-hydroxy-5beta-steroid:NADP⁺ 3-oxidoreductase. Other names in common use include 3beta-hydroxysteroid 5beta-oxidoreductase, and 3beta-hydroxysteroid 5beta-progesterone oxidoreductase.

References

• Stuhlemmer U, Kreis W (1996). "Cardenolide formation and activity of pregnane-modifying enzymes in cell suspension cultures, shoot cultures and leaves of Digitalis lanata". Plant Physiol. Biochem. 34: 85–91.
• Seitz HU, Gaertner DE (1994). "Enzymes in cardenolide-accumulating shoot cultures of Digitalis purpurea". Plant Cell. 38: 337–344. doi:10.1007/bf00033894.
• Lindemann P, Luckner M (1997). "Biosynthesis of pregnane derivatives in somatic embryos of Digitalis lanata". Phytochemistry. 46 (3): 507–513. doi:10.1016/S0031-9422(97)00315-4.