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- Animals
- Bat, gray (Myotis grisescens) E
- Bat, Indiana (Myotis sodalis) E
- Bat, Virginia big-eared (Corynorhinus (=Plecotus) townsendii virginianus) E
- Clubshell Entire Range; Except where listed as Experimental Populations (Pleurobema clava) E
- Fanshell (Cyprogenia stegaria) E
- Mucket, pink (pearlymussel) (Lampsilis abrupta) E
- Riffleshell, northern (Epioblasma torulosa rangiana) E
- Salamander, Cheat Mountain (Plethodon nettingi) T
- Snail, flat-spired three-toothed (Triodopsis platysayoides) T
- Spinymussel, James (Pleurobema collina) E
- Beetle, American burying (Nicrophorus americanus) E
- Puma (=cougar), eastern (Puma (=Felis) concolor couguar) E
- Ring pink (mussel) (Obovaria retusa) E
- Wolf, gray Lower 48 States, except where delisted and where EXPN. Mexico. (Canis lupus) E
- Blossom, tubercled (pearlymussel) Entire Range; Except where listed as Experimental Populations (Epioblasma torulosa torulosa) E
- Isopod, Madison Cave (Antrolana lira) T
- Plants
- Bulrush, Northeastern (Scirpus ancistrochaetus) E
- Clover, running buffalo (Trifolium stoloniferum) E
- Harperella (Ptilimnium nodosum) E
- Pogonia, small whorled (Isotria medeoloides)T
- Rock-cress, shale barren (Arabis serotina) E
- Spiraea, Virginia (Spiraea virginiana) T
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