ORC5

Origin recognition complex subunit 5 is a protein that in humans is encoded by the ORC5 (ORC5L) gene.^[5][6][7]

ORC5
Identifiers
Aliases	ORC5, ORC5L, ORC5P, ORC5T, PPP1R117, origin recognition complex subunit 5
External IDs	OMIM: 602331; MGI: 1347044; HomoloGene: 37636; GeneCards: ORC5; OMA:ORC5 - orthologs
Gene location (Human) Chr. Chromosome 7 (human)[1] Band 7q22.1-q22.2 Start 104,126,341 bp[1] End 104,208,047 bp[1]
Gene location (Mouse) Chr. Chromosome 5 (mouse)[2] Band 5|5 A3 Start 22,691,483 bp[2] End 22,755,427 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in secondary oocyte endothelial cell gonad testicle middle temporal gyrus buccal mucosa cell Brodmann area 23 ventricular zone Achilles tendon body of pancreas Top expressed in primary oocyte secondary oocyte zygote spermatocyte epiblast fetal liver hematopoietic progenitor cell primitive streak spermatid endocardial cushion embryo More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding DNA replication origin binding protein binding ATP binding molecular function Cellular component nuclear origin of replication recognition complex origin recognition complex nucleus nucleoplasm cytosol Biological process G1 phase DNA replication DNA replication initiation G1/S transition of mitotic cell cycle Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5001 26429 Ensembl ENSG00000164815 ENSMUSG00000029012 UniProt O43913 Q9WUV0 RefSeq (mRNA) NM_001197292 NM_002553 NM_181747 NM_011959 RefSeq (protein) NP_002544 NP_859531 NP_036089 Location (UCSC) Chr 7: 104.13 – 104.21 Mb Chr 5: 22.69 – 22.76 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown to form a core complex with ORC2L, -3L, and 4L. Alternatively spliced transcript variants encoding distinct isoforms have been described.^[7]

Interactions

ORC5 has been shown to interact with:

• MCM2,^[8]
• MCM3,^[8]
• MCM4,^[8]
• MCM7,^[8]
• ORC1,^[8]
• ORC2,^{[8][9][10][11]}
• ORC3,^{[8][10][11][12]} and
• ORC4.^[8][9][10]

References

1. GRCh38: Ensembl release 89: ENSG00000164815 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000029012 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Ishiai M, Dean FB, Okumura K, Abe M, Moon KY, Amin AA, Kagotani K, Taguchi H, Murakami Y, Hanaoka F, O'Donnell M, Hurwitz J, Eki T (Dec 1997). "Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22". Genomics. 46 (2): 294–8. doi:10.1006/geno.1997.5003. PMID 9417919.
6. Tugal T, Zou-Yang XH, Gavin K, Pappin D, Canas B, Kobayashi R, Hunt T, Stillman B (Dec 1998). "The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p". The Journal of Biological Chemistry. 273 (49): 32421–9. doi:10.1074/jbc.273.49.32421. PMID 9829972.
7. "Entrez Gene: ORC5L origin recognition complex, subunit 5-like (yeast)".
8. Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". Journal of Molecular Biology. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612.
9. Quintana DG, Thome KC, Hou ZH, Ligon AH, Morton CC, Dutta A (Oct 1998). "ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases". The Journal of Biological Chemistry. 273 (42): 27137–45. doi:10.1074/jbc.273.42.27137. PMID 9765232.
10. Dhar SK, Delmolino L, Dutta A (Aug 2001). "Architecture of the human origin recognition complex". The Journal of Biological Chemistry. 276 (31): 29067–71. doi:10.1074/jbc.M103078200. PMID 11395502.
11. Vashee S, Simancek P, Challberg MD, Kelly TJ (Jul 2001). "Assembly of the human origin recognition complex". The Journal of Biological Chemistry. 276 (28): 26666–73. doi:10.1074/jbc.M102493200. PMID 11323433.
12. Pinto S, Quintana DG, Smith P, Mihalek RM, Hou ZH, Boynton S, Jones CJ, Hendricks M, Velinzon K, Wohlschlegel JA, Austin RJ, Lane WS, Tully T, Dutta A (May 1999). "latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant". Neuron. 23 (1): 45–54. doi:10.1016/s0896-6273(00)80752-7. PMID 10402192. S2CID 781511.

Further reading

• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Klemm RD, Austin RJ, Bell SP (Feb 1997). "Coordinate binding of ATP and origin DNA regulates the ATPase activity of the origin recognition complex". Cell. 88 (4): 493–502. doi:10.1016/S0092-8674(00)81889-9. PMID 9038340. S2CID 12927727.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Quintana DG, Thome KC, Hou ZH, Ligon AH, Morton CC, Dutta A (Oct 1998). "ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases". The Journal of Biological Chemistry. 273 (42): 27137–45. doi:10.1074/jbc.273.42.27137. PMID 9765232.
• Jiang W, McDonald D, Hope TJ, Hunter T (Oct 1999). "Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication". The EMBO Journal. 18 (20): 5703–13. doi:10.1093/emboj/18.20.5703. PMC 1171637. PMID 10523313.
• Thome KC, Dhar SK, Quintana DG, Delmolino L, Shahsafaei A, Dutta A (Nov 2000). "Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins". The Journal of Biological Chemistry. 275 (45): 35233–41. doi:10.1074/jbc.M005765200. PMID 10954718.
• Vashee S, Simancek P, Challberg MD, Kelly TJ (Jul 2001). "Assembly of the human origin recognition complex". The Journal of Biological Chemistry. 276 (28): 26666–73. doi:10.1074/jbc.M102493200. PMID 11323433.
• Fröhling S, Nakabayashi K, Scherer SW, Döhner H, Döhner K (Apr 2001). "Mutation analysis of the origin recognition complex subunit 5 (ORC5L) gene in adult patients with myeloid leukemias exhibiting deletions of chromosome band 7q22". Human Genetics. 108 (4): 304–9. doi:10.1007/s004390100498. PMID 11379876. S2CID 23879624.
• Dhar SK, Delmolino L, Dutta A (Aug 2001). "Architecture of the human origin recognition complex". The Journal of Biological Chemistry. 276 (31): 29067–71. doi:10.1074/jbc.M103078200. PMID 11395502.
• Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". Journal of Molecular Biology. 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. PMID 12614612.
• Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau AY, Walter JC, LaBaer J (Jul 2004). "Self-assembling protein microarrays". Science. 305 (5680): 86–90. Bibcode:2004Sci...305...86R. doi:10.1126/science.1097639. PMID 15232106. S2CID 20936301.