Lacuna (plural lacunas or lacunae) may refer to:
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lacuna in Wiktionary, the free dictionary.
- Lacuna (manuscripts), a gap in a manuscript, inscription, text, painting, or musical work
- Great Lacuna, a lacuna of eight leaves in the Codex Regius where there was heroic Old Norse poetry
- Lacuna (music), an intentional, extended passage in a musical work during which no notes are played
- Scientific lacuna, an area of science that has not been studied but has potential to be studied
- Lacuna or accidental gap, in linguistics, a word that does not exist but which would be permitted by the rules of a language
- Lacuna, in law, largely overlapping a non liquet ("it is not clear"), a gap (in the law)
- Lacuna (comics), a fictional Marvel Comics character
- Lacuna (film), a 2012 Chinese romantic comedy film
- Lacuna (gastropod), a genus of sea snails in the family Littorinidae
- Helcogramma lacuna (H. lacuna), a species of fish in the genus Helcogramma
- Mallomonas lacuna (M. lacuna), a species of heterokont algae
- Lacuna Island, Antarctica
- Jessie Lacuna (born 1993), a Filipino swimmer
- The Lacuna, a 2009 novel by Barbara Kingsolver
- Lacuna, Inc., a fictional company in the 2004 film Eternal Sunshine of the Spotless Mind
- Lacuna, the name of several lakes of Titan, the moon of Saturn