Killer bees most often refers to Africanized bees, a hybrid of the African honey bee with various European honey bees.
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Killer bees or Killer B's may also refer to:
- Killer Bee (band), a Swedish-Canadian rock band formed by Anders Rönnblom and Brian Frank
- Wu-Tang Killa Beez, a collective nickname for affiliates of the Wu-Tang Clan rap group
- The Killer Bees, an American funk/soul/rock band co-founded by Papa Mali
- WXKB, a Top 40 music radio station in Cape Coral, Florida nicknamed "The Killer B"
- DXKB, a Philippine radio station, formerly nicknamed "Killer Bee"
- Bridgehampton School, nickname of high school basketball team featured in 2017 film documentary.
- The Killer Bees (professional wrestling), a professional wrestling tag team
- Killer B's (Houston Astros), collective nickname for six Houston Astros players in the 1990s and early 2000s
- Killer B's (Miami), collective nickname for eight Miami Dolphins defensive players in the 1980s
- Rio Grande Valley Killer Bees (NAHL), a former North American Hockey League team based in Hidalgo, Texas, US
- Rio Grande Valley Killer Bees (CHL), a former Central Hockey League team based in Hidalgo, Texas, US
- Group B, a 1980s class of World Rally Championship cars nicknamed "Killer B's" due to their poor safety record
- Killer B's (Pittsburgh), a nickname for three players for the 2013–2017 Pittsburgh Steelers: Ben Roethlisberger, Antonio Brown, and Le'Veon Bell
- Killer Bees!, a 1983 video game for the Magnavox Odyssey2
- Cammy, a character in the Street Fighter video-game series sometimes called Killer Bee
- Killer bees (business), firms or individuals employed by a target company to fend off a takeover bid
- Killer Bees (Texas Senate), a group of Texas senators, including Glenn Kothmann, who in 1979 went into hiding to prevent a quorum
- Killer Beez, a gang in New Zealand