Isobaric tag for relative and absolute quantitation
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Isobaric tags for relative and absolute quantitation (iTRAQ) is an isobaric labeling method used in quantitative proteomics by tandem mass spectrometry to determine the amount of proteins from different sources in a single experiment.[1][2][3] It uses stable isotope labeled molecules that can be covalent bonded to the N-terminus and side chain amines of proteins.