File:MultiPhotonExcitation-Fig10-doi10.1186slash1475-925X-5-36-clipping.JPEG
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MultiPhotonExcitation-Fig10-doi10.1186slash1475-925X-5-36-clipping.JPEG (714 × 467 pixels, file size: 81 KB, MIME type: image/jpeg)
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DescriptionMultiPhotonExcitation-Fig10-doi10.1186slash1475-925X-5-36-clipping.JPEG |
English: Original figure legend: Multiple fluorescence 2PE imaging. 2PE multiple fluorescence image from a 16 μm cryostat section of mouse intestine stained with a combination of fluorescent stains (F-24631, Molecular Probes). Alexa Fluor 350 wheat germ agglutinin, a blue-fluorescent lectin, was used to stain the mucus of goblet cells. The filamentous actin prevalent in the brush border was stained with red-fluorescent Alexa Flu or 568 phalloidin. Finally, the nuclei were stained with SYTOX ® Green nucleic acid stain. Imaging has been performed at 780 nm, 100 x 1.4 NA Leica objective, using a Chameleon XR ultrafast Ti-Sapphire laser (Coherent Inc., USA) coupled at LAMBS-MicroScoBio with a Spectral Confocal Laser Scanning Microscope, Leica SP2-AOBS.
Deutsch: Zweiphotonenaufnahme an einem Schnitt durch einen Mausdarm. Zellkerne in grün, Schleim der Becherzellen in blau, Aktin (Phalloidin-Färbung) in rot. Anregung erfolgte bei 780 nm durch einen Titan:Saphir-Laser.
Français : légende originale de l'image : imagerie en fluorescenc emultiple 2PE d'une section de 16 µm de cryostat d'intestin de souris coloré avec une combinaison de colorants fluorescents (F-24631, Molecular Probes). l'Alexa Fluor 350 d'agglutinine degerme de blé, une lectine bleu fluorescente, a été utilisée pour colorer le mucus des cellules caliciformes. L'actine filamenteuse a été colorée avec du rouge fluorescent (Alexa Flu ou phalloïdine 568). Enfin, les noyaux ont été colorés avec un autre colorant (SYTOX ® Green nucleic acid stain). L'image a été faite à 780 nm, avec un objectif Leica 100 x 1,4 NA, en utilisant un éclairage laser (Chameleon XR ultrafast Ti-Sapphire laser (Coherent Inc., USA) ) couplé à un microscope LAMBS-MicroScoBio (Spectral Confocal Laser Scanning Microscope, Leica SP2-AOBS). |
Date | Original version: 6 June 2006. Clipping: 4. March 2009. |
Source |
Multi-photon excitation microscopy. BioMedical Engineering OnLine, 2006, 5:36. |
Author |
Alberto Diaspro, Paolo Bianchini, Giuseppe Vicidomini, Mario Faretta, Paola Ramoino and Cesare Usai. |
Permission (Reusing this file) |
This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Author | TCS User |
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Orientation | Normal |
Horizontal resolution | 72 dpi |
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Software used | Adobe Photoshop 7.0 |
File change date and time | 21:47, 4 March 2009 |
Y and C positioning | Centered |
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