Eustachius may refer to:
- Variant of the name Eustathius
- Saint Eustachius
- Sint Eustatius, Dutch island in the Caribbean
- Bartolomeo Eustachi (c. 1500–1510 – 1574), anatomist
- Eustáquio van Lieshout (1890–1943), Dutch missionary in Brazil
- Eustachius Kugler, beatified German religious
- Eustochius, fifth bishop of Tours from 443 to 460
- Eustathius, son of Macrobius
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