Diacylglycerol—sterol O-acyltransferase

In enzymology, a diacylglycerol-sterol O-acyltransferase (EC 2.3.1.73) is an enzyme that catalyzes the chemical reaction

1,2-diacyl-sn-glycerol + sterol ${\displaystyle \rightleftharpoons }$ monoacylglycerol + sterol ester

diacylglycerol-sterol O-acyltransferase
Identifiers
EC no.	2.3.1.73
CAS no.	79586-23-5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Thus, the two substrates of this enzyme are 1,2-diacyl-sn-glycerol and sterol, whereas its two products are monoacylglycerol and sterol ester.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is 1,2-diacyl-sn-glycerol:sterol O-acyltransferase. This enzyme is also called 1,2-diacyl-sn-glycerol:sterol acyl transferase. This enzyme participates in bile acid biosynthesis.

References

• Bartlett K, Keat MJ, Mercer EI (1974). "Biosynthesis of sterol esters in Phycomyces blakesleeanus". Phytochemistry. 13 (7): 1107–1113. doi:10.1016/0031-9422(74)80083-X.
• Garcia RE, Mudd JB (1978). "Metabolism of monoacylglycerol and diacylglycerol by enzyme preparations from spinach leaves". Arch. Biochem. Biophys. 191 (2): 487–93. doi:10.1016/0003-9861(78)90387-9. PMID 742884.
• Garcia RE; Mudd JB (1981). "1,2-Diacyl-sn-glycerol: Sterol acyl transferase from spinach leaves (Spinacia olerecea L.)". 1,2-Diacyl-sn-glycerol:sterol acyl transferase from spinach leaves (Spinacia oleracea L.). Methods Enzymol. Vol. 71. pp. 768–772. doi:10.1016/0076-6879(81)71090-5. ISBN 978-0-12-181971-2.