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3,3',5,5'-Tetramethylbenzidine
Chemical compound / From Wikipedia, the free encyclopedia
3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).[1] TMB is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights. Used to detect hematuria as it turns blue in contact with hemoglobin.
Quick Facts Names, Identifiers ...
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Names | |
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Preferred IUPAC name
3,3′,5,5′-Tetramethyl[1,1′-biphenyl]-4,4′-diamine | |
Other names
3,3′,5,5′-Tetramethylbiphenyl-4,4'-diamine | |
Identifiers | |
3D model (JSmol) |
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ChEMBL | |
ChemSpider |
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ECHA InfoCard | 100.053.949 ![]() |
EC Number |
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PubChem CID |
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UNII | |
CompTox Dashboard (EPA) |
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Properties | |
C16H20N2 | |
Molar mass | 240.3482 g/mol |
Melting point | 168 to 171 °C (334 to 340 °F; 441 to 444 K) |
Hazards | |
GHS labelling: | |
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Warning | |
H302, H315, H319, H335 | |
P261, P264, P270, P271, P280, P301+P312, P302+P352, P304+P340, P305+P351+P338, P312, P321, P330, P332+P313, P337+P313, P362, P403+P233, P405, P501 | |
Safety data sheet (SDS) | External MSDS |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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