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I biokemi er en isomerase et enzym, der katalyserer den strukturelle omdannelse af isomerer. Isomeraser katalyserer således reaktioner på formen
hvor A og B er hinandens isomerer.
Isomerasers navne dannes som "substratisomerase" (for eksempel glukose-6-fosfatisomerase), eller som "substrat-type af isomerase" (for eksempel fosfoglukomutase).
Isomeraser har deres egne EC-numre i enzymklassifikationssystemet: EC 5. Isomeraser kan yderligere klassificeres i seks underklasser:
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